用于检测结直肠癌的粪便DNA完整性方法。

Stool DNA Integrity Method for Colorectal Cancer Detection.

作者信息

Rengucci Claudia, De Maio Giulia, Menghi Maura, Calistri Daniele

机构信息

Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Forlì-Cesena, Italy.

Diatech Pharmacogenetics, Jesi, Ancona, Italy.

出版信息

Methods Mol Biol. 2018;1765:193-202. doi: 10.1007/978-1-4939-7765-9_12.

DOI:10.1007/978-1-4939-7765-9_12

PMID:29589309

Abstract

Fluorescence long DNA (FL-DNA) is a non-invasive and simple-to-perform stool DNA test. This assay consists of a qualitative and quantitative real-time PCR (RT PCR) analysis. FL-DNA has great potential in colorectal cancer (CRC) lesions detection used alone or in combination with the standard CRC screening tool: immunochemical fecal occult blood test (iFOBT).

摘要

荧光长链DNA（FL-DNA）检测是一种非侵入性且操作简便的粪便DNA检测方法。该检测包括定性和定量实时聚合酶链反应（RT-PCR）分析。FL-DNA在单独用于或与标准的结直肠癌（CRC）筛查工具——免疫化学粪便潜血试验（iFOBT）联合用于CRC病变检测方面具有巨大潜力。