Oljira Temesgen, Muleta Diriba, Jida Mulissa
Microbiology and Microbial Biotechnology Graduate Program, Department of Biology, Wollega University, Nekemte, Ethiopia.
Industrial Biotechnology Unit, Department of Biotechnology, Wolkite University, Wolkite, Ethiopia.
Biotechnol Res Int. 2018 Feb 11;2018:9745198. doi: 10.1155/2018/9745198. eCollection 2018.
Biological wastewater treatment is economically feasible and ecofriendly. This study was aimed at isolating bacteria from brewery wastes and evaluating their bioremediation potential as individual isolate and/or their consortium in reducing the pollutants of brewery effluents. A total of 40 bacterial isolates were recovered and of these the three best isolates were selected. The selected bacteria were identified to genus level by using morphological and biochemical characteristics. Accordingly, the isolates were identified as sp., sp., and sp. After 12 days of incubation, the removal efficiency of these three isolates and their combinations for biological oxygen demand and chemical oxygen demand varied from 73.55% to 94.85% and 76.78% to 93.25%, respectively. Total nitrogen and phosphorus removal was within the range of 54.43% to 77.21% and 41.80% to 78.18%, respectively. Total suspended solid, total solid, and total dissolved solids removal ranged from 66.74% to 90.3%, 54.69% to 88.5%, and 53.02% to 88.2%, respectively. The pH and electrical conductivity values ranged from 6.81 to 8.65 and 3.31 mS/cm to 3.67 mS/cm, respectively. The treated effluent increased seeds germination from 80% to 100%, with mean germination time of 3.1 to 5.2 days and seedlings length of 2.3 cm to 6.3 cm. Therefore, the development of this finding into a large scale offers an attractive technology for brewery waste treatment.
生物废水处理在经济上可行且环保。本研究旨在从啤酒厂废物中分离细菌,并评估它们作为单一菌株和/或其联合体在减少啤酒厂废水污染物方面的生物修复潜力。共回收了40株细菌分离株,从中挑选出三株最佳分离株。利用形态学和生化特征将所选细菌鉴定到属水平。据此,这些分离株被鉴定为 属、 属和 属。培养12天后,这三株分离株及其组合对生物需氧量和化学需氧量的去除效率分别在73.55%至94.85%和76.78%至93.25%之间。总氮和总磷的去除率分别在54.43%至77.21%和41.80%至78.18%之间。总悬浮固体、总固体和总溶解固体的去除率分别在66.74%至90.3%、54.69%至88.5%和53.02%至88.2%之间。pH值和电导率值分别在6.81至8.65和3.31 mS/cm至3.67 mS/cm之间。处理后的废水使种子发芽率从80%提高到100%,平均发芽时间为3.1至5.2天,幼苗长度为2.3 cm至6.3 cm。因此,将这一研究成果扩大到大规模应用为啤酒厂废水处理提供了一项有吸引力的技术。