糖尿病母亲的大鼠后代青春期前卵巢组织形态测量学的改变。
Alterations of the ovarian histomorphometry at pre-puberty in rat offspring from diabetic mothers.
作者信息
Khaksar Zabihollah, Jelodar Gholamali, Hematian Hooman, Poorahmadi Mohamad
机构信息
Department of Basic Sciences, School of Veterinary Medicine Shiraz University Shiraz Iran.
Department of Veterinary Sciences, Yasooj Branch Islamic Azad University Yasooj Iran.
出版信息
Reprod Med Biol. 2013 May 14;12(4):173-178. doi: 10.1007/s12522-013-0151-3. eCollection 2013 Oct.
PURPOSE
Maternal diabetes leads to increased blood glucose concentration in the mother and consequently in the foetus, causing various neonatal problems. This study was conducted to evaluate the effects of maternal diabetes on foetal ovarian structure.
METHODS
Sixteen adult female rats were allocated into two equal groups. Diabetes was induced in one group by alloxan. Both groups became pregnant by natural mating. Thirty days after birth, the female offspring were terminated, the body weight and blood glucose of the animals measured and their ovaries removed. Various histological and cellular parameters were determined using histological and electron microscopy techniques.
RESULTS
Results revealed a significant increase in body weight and blood glucose in the offspring of the diabetic mothers (ODM) compared to that of the controls. The weight, volume and diameter of the ovary and the ovarian capsule thickness were inclined to decrease in ODM compared to that of controls. The number and diameter of primary, pre-antral and antral follicles were decreased in ovaries in the ODM. The electro-micrographs have demonstrated the organelle alterations in oocytes and granulosa cells that suggest the apoptosis progress and oxidative stress.
CONCLUSIONS
Maternal hyperglycaemia exhibited deleterious effects on the female reproductive system in the offspring.
目的
母体糖尿病会导致母亲血糖浓度升高，进而使胎儿血糖浓度升高，引发各种新生儿问题。本研究旨在评估母体糖尿病对胎儿卵巢结构的影响。
方法
将16只成年雌性大鼠平均分为两组。其中一组通过四氧嘧啶诱导糖尿病。两组均通过自然交配怀孕。出生30天后，处死雌性后代，测量动物体重和血糖，并摘除其卵巢。使用组织学和电子显微镜技术确定各种组织学和细胞参数。
结果
结果显示，与对照组相比，糖尿病母亲的后代（ODM）体重和血糖显著增加。与对照组相比，ODM的卵巢重量、体积和直径以及卵巢囊厚度倾向于减小。ODM卵巢中的初级卵泡、窦前卵泡和窦卵泡数量及直径均减少。电子显微镜照片显示卵母细胞和颗粒细胞中的细胞器改变，提示细胞凋亡进程和氧化应激。
结论
母体高血糖对后代雌性生殖系统有有害影响。
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