Baer R, Boehm T, Yssel H, Spits H, Rabbitts T H
Laboratory of Molecular Biology, Cambridge, UK.
EMBO J. 1988 Jun;7(6):1661-8. doi: 10.1002/j.1460-2075.1988.tb02993.x.
We have examined DNA rearrangements within a 120 kb cloned region of the human T cell receptor J delta-C delta/J alpha-C alpha locus. Three types of pattern emerge from an analysis of T cell lines and clones. Firstly, cells with two rearrangements within J delta-C delta; secondly, cells with one rearrangement within J delta-C delta and one or more J alpha rearrangements, and finally, cells with rearrangements within J alpha and consequential deletion of the delta locus. Further analysis by cloning of rearrangements within the J alpha locus show that, in addition to V alpha-J alpha joins, J alpha-J alpha aberrant recombinations occur and rearrangement data indicate that such events are frequent. A model is presented to account for such recombinations.
我们已经检测了人类T细胞受体Jδ-Cδ/Jα-Cα基因座120kb克隆区域内的DNA重排。对T细胞系和克隆进行分析后出现了三种类型的模式。首先，在Jδ-Cδ内有两种重排的细胞；其次，在Jδ-Cδ内有一重排且有一个或多个Jα重排的细胞，最后，在Jα内有重排且δ基因座随之缺失的细胞。通过克隆Jα基因座内的重排进行的进一步分析表明，除了Vα-Jα连接外，还发生Jα-Jα异常重组，重排数据表明此类事件很常见。本文提出了一个模型来解释此类重组。
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