采用深共晶溶剂从 中提取、纯化新型多糖及其抗氧化活性研究。
Extraction, purification and antioxidant activity of novel polysaccharides from by deep eutectic solvents.
机构信息
Laboratory of Applied Biocatalysis, South China University of Technology , Guangzhou , China.
Guangdong Provincial Key Laboratory of Silviculture, Protection and Utlization , Guangzhou , China.
出版信息
Nat Prod Res. 2019 Nov;33(22):3248-3253. doi: 10.1080/14786419.2018.1471480. Epub 2018 May 4.
Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography. Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated. Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2.2:1 and 3.7:1, respectively. Structural features of DOP1-DES and DOP2-DES were investigated by a combination of FT-IR, methylation analysis and NMR, which indicated that both polysaccharides had a similarly backbone consisting of (1, 4)-linked -D-Man and (1, 4)-linked -D-Glc with different ratio. Simultaneously, the two fractions possessed considerable antioxidant activity.
首次采用 DES 从 中提取到两种新型多糖 DOP1-DES 和 DOP2-DES，分子量分别为 297911 Da 和 30400 Da，并通过大孔树脂 AB-8、Sephadex G75 和 Sephadex G200 层析进行纯化。此外，还考察了提取温度、DES 摩尔比、反应时间和复合酶浓度对多糖提取率的影响。单糖组成分析表明，DOP1-DES 和 DOP2-DES 分别由 D-葡萄糖和 D-甘露糖组成，比例为 2.2:1 和 3.7:1。通过 FT-IR、甲基化分析和 NMR 的结合，研究了 DOP1-DES 和 DOP2-DES 的结构特征，结果表明两种多糖均具有类似的由（1,4）-连接的 -D-Man 和（1,4）-连接的 -D-Glc 组成的主链，但其比例不同。同时，这两个部分均具有相当的抗氧化活性。
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