Viegas Carla, Moreira Ricardo, Faria Tiago, Caetano Liliana Aranha, Carolino Elisabete, Gomes Anita Quintal, Viegas Susana
H&TRC- Health & Technology Research Center, ESTeSL- Escola Superior de Tecnologia da Saúde, Instituto Politécnico de Lisboa, Lisbon, Portugal.
Centro de Investigação em Saúde Pública, Escola Nacional de Saúde Pública, Universidade NOVA de Lisboa.
Arch Environ Occup Health. 2019;74(6):341-349. doi: 10.1080/19338244.2018.1472545. Epub 2018 Jul 10.
The frequency and importance of infections is increasing worldwide. This study aimed to assess the occupational exposure of forklifts and taxi drivers to spp. Nineteen filters from air conditioning system of taxis, 17 from forklifts and 37 from personal vehicles were assessed. Filters extract were streaked onto MEA, DG18 and in azole-supplemented media. Real-time quantitative PCR amplification of selected species-complex was also performed. Forklifts filter samples presented higher median values. section was the most observed in forklifts filters in MEA (28.2%) and in azole-supplemented media. DNA from sections and was successfully amplified by qPCR. This study enlightens the added value of using filters from the air conditioning system to assess spp. occupational exposure. azole resistance screening should be included in future occupational exposure assessments.
感染的频率和重要性在全球范围内不断增加。本研究旨在评估叉车司机和出租车司机对 spp. 的职业暴露情况。对来自出租车空调系统的19个过滤器、来自叉车的17个过滤器和来自个人车辆的37个过滤器进行了评估。将过滤器提取物划线接种到MEA、DG18和含唑补充培养基上。还对选定的物种复合体进行了实时定量PCR扩增。叉车过滤器样本的中位数较高。在MEA(28.2%)和含唑补充培养基中,叉车过滤器中观察到的 节段最多。通过qPCR成功扩增了 节段和 的DNA。本研究揭示了使用空调系统过滤器评估 spp. 职业暴露的附加价值。未来的职业暴露评估应包括唑类耐药性筛查。