Department of Ophthalmology, School of Medicine, Shimane University, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
Department of Research and Development, Kotobuki Seika Co., Ltd., 2028 Hatagasaki, Yonago, Tottori 683-0845, Japan.
Nutrients. 2018 May 10;10(5):593. doi: 10.3390/nu10050593.
Retinal tissue is exposed to oxidative stress caused by visible light. Light-damaged rat used in age-related macular degeneration (AMD) studies clarified that antioxidants decrease retinal light damage. Albino rats were exposed to 5000 Lux light for 12 h with oral administration of the polyphenolic compounds fraction (PF) from the seed shells of Japanese horse chestnut (30 mg/kg, 100 mg/kg, and 300 mg/kg body weight: BW). To evaluate the protective effects against light damage, electroretinograms (ERGs), the outer nuclear layer (ONL) thickness, the antioxidant activity of plasma, oxidized retinal lipids, and the detection of apoptosis were examined. To reveal their active compounds, PF were separated into an A-type proanthocyanidin (PAF) and a flavonol -glycosides fraction. The protective effects of these fractions against light damage were compared by measuring the thickness of the ERGs and ONL. Compared with the negative control, the PF group (100 mg/kg and 300 mg/kg BW) significantly suppressed the decrease of the ERG amplitudes and ONL thickness. PF (300 mg/kg BW) induced the elevation of in vivo antioxidant activity, and the suppression of retinal lipid oxidation. PF administration also suppressed apoptotic cell death. The protective effects against light damage were attributable to the antioxidant activity of PAF. The light-induced damage of retinas was protected by oral administration of PF and PAF. Taken together, these compounds are potentially useful for the prevention of the disease caused by light exposure.
The protective effects of retinal damage by light exposure were evaluated using polyphenolic compounds from the seed shells of Japanese horse chestnut ( BLUME) as an antioxidant. Decreases in the electroretinographic amplitude and outer nuclear layer thickness were suppressed by the polyphenolic compounds of the seed shells. Polyphenolic compounds from the seed shells of Japanese horse chestnut inhibited the oxidation of retinal lipids. Highly polymeric A-type proanthocyanidin from the seed shells protected the rat retina from light exposure damage by inhibiting oxidative stress and apoptotic mechanisms.
视网膜组织会受到可见光引起的氧化应激。用于年龄相关性黄斑变性（AMD）研究的光损伤大鼠阐明了抗氧化剂可减少视网膜光损伤。白化大鼠接受 5000 Lux 光照 12 小时，并口服来自日本七叶树（Aesculus turbinata BLUME）种壳的多酚化合物（PF），剂量分别为 30mg/kg、100mg/kg 和 300mg/kg 体重。为了评估对光损伤的保护作用，检查了视网膜电图（ERG）、外核层（ONL）厚度、血浆抗氧化活性、氧化视网膜脂质和凋亡检测。为了揭示其活性化合物，将 PF 分离为 A 型原花青素（PAF）和黄酮醇糖苷。通过测量 ERG 和 ONL 的厚度比较这些级分对光损伤的保护作用。与阴性对照相比，PF 组（100mg/kg 和 300mg/kg BW）显著抑制 ERG 幅度和 ONL 厚度的降低。PF（300mg/kg BW）诱导体内抗氧化活性升高，抑制视网膜脂质氧化。PF 给药还抑制了细胞凋亡。对光损伤的保护作用归因于 PAF 的抗氧化活性。口服 PF 和 PAF 可保护视网膜免受光诱导损伤。总之，这些化合物可能对预防由光暴露引起的疾病有用。
使用来自日本七叶树（BLUME）种壳的多酚化合物作为抗氧化剂评估光暴露引起的视网膜损伤的保护作用。光损伤的电视网膜图幅度和外核层厚度的降低被种壳的多酚化合物抑制。来自日本七叶树种壳的多酚化合物抑制视网膜脂质的氧化。来自种壳的高分子量 A 型原花青素通过抑制氧化应激和凋亡机制保护大鼠视网膜免受光暴露损伤。
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