Department of Periodontology, College of Dental Medicine, University of Bern, Bern, Switzerland; Cell Therapy Institute, Centre for Collaborative Research, Nova Southeastern University, Fort Lauderdale, FL, USA.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Acta Biomater. 2018 Jul 15;75:35-51. doi: 10.1016/j.actbio.2018.05.021. Epub 2018 May 15.
There is currently widespread interest within the biomaterial field to locally deliver biomolecules for bone and cartilage regeneration. Substantial work to date has focused on the potential role of these biomolecules during the healing process, and the carrier system utilized is a key factor in their effectiveness. Platelet rich fibrin (PRF) is a naturally derived fibrin scaffold that is easily obtained from peripheral blood following centrifugation. Slower centrifugation speeds have led to the commercialization of a liquid formulation (liquid-PRF) resulting in an upper plasma layer composed of liquid fibrinogen/thrombin prior to clot formation that remains in its liquid phase for approximately 15 min until injected into bodily tissues. Herein, we introduce the use of liquid PRF as an advanced local delivery system for small and large biomolecules. Potential target molecules including large (growth factors/cytokines and morphogenetic/angiogenic factors), as well as small (antibiotics, peptides, gene therapy and anti-osteoporotic) molecules are considered potential candidates for enhanced bone/cartilage tissue regeneration. Furthermore, liquid-PRF is introduced as a potential carrier system for various cell types and nano-sized particles that are capable of limiting/by-passing the immune system and minimizing potential foreign body reactions within host tissues following injection.
There is currently widespread interest within the biomaterial field to locally deliver biomolecules for bone and cartilage regeneration. This review article focuses on the use of a liquid version of platelet rich fibrin (PRF) composed of liquid fibrinogen/thrombin as a drug delivery system. Herein, we introduce the use of liquid PRF as an advanced local delivery system for small and large biomolecules including growth factors, cytokines and morphogenetic/angiogenic factors, as well as antibiotics, peptides, gene therapy and anti-osteoporotic molecules as potential candidates for enhanced bone/cartilage tissue regeneration.
目前，生物材料领域广泛关注局部递送生物分子以促进骨和软骨再生。迄今为止，大量工作集中在这些生物分子在愈合过程中的潜在作用上，而所用的载体系统是其有效性的关键因素。富含血小板的纤维蛋白 (PRF) 是一种天然衍生的纤维蛋白支架，可通过离心从外周血中轻松获得。较慢的离心速度导致商业化的液体配方（液体-PRF）的产生，在上血浆层由液体纤维蛋白原/凝血酶组成，在形成凝块之前处于液体相，持续约 15 分钟，直到注入身体组织。在此，我们介绍将液体 PRF 用作小分子和大分子的先进局部递送系统。包括大（生长因子/细胞因子和形态发生/血管生成因子）和小（抗生素、肽、基因治疗和抗骨质疏松）分子在内的潜在靶分子被认为是增强骨/软骨组织再生的潜在候选物。此外，液体-PRF 被引入为各种细胞类型和纳米级颗粒的载体系统，这些颗粒能够限制/绕过免疫系统并在注入后最小化宿主组织中的潜在异物反应。
目前，生物材料领域广泛关注局部递送生物分子以促进骨和软骨再生。本文综述了使用富含血小板的纤维蛋白（PRF）的液体版本（由液体纤维蛋白原/凝血酶组成）作为药物递送系统。在此，我们介绍了将液体 PRF 用作小分子和大分子的先进局部递送系统的用途，包括生长因子、细胞因子和形态发生/血管生成因子以及抗生素、肽、基因治疗和抗骨质疏松分子作为增强骨/软骨组织再生的潜在候选物。
