Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
SIB Swiss Institute of Bioinformatics, Biozentrum, University of Basel, Klingelbergstrasse 50-70, CH-4056 Basel, Switzerland.
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303. doi: 10.1093/nar/gky427.
Homology modelling has matured into an important technique in structural biology, significantly contributing to narrowing the gap between known protein sequences and experimentally determined structures. Fully automated workflows and servers simplify and streamline the homology modelling process, also allowing users without a specific computational expertise to generate reliable protein models and have easy access to modelling results, their visualization and interpretation. Here, we present an update to the SWISS-MODEL server, which pioneered the field of automated modelling 25 years ago and been continuously further developed. Recently, its functionality has been extended to the modelling of homo- and heteromeric complexes. Starting from the amino acid sequences of the interacting proteins, both the stoichiometry and the overall structure of the complex are inferred by homology modelling. Other major improvements include the implementation of a new modelling engine, ProMod3 and the introduction a new local model quality estimation method, QMEANDisCo. SWISS-MODEL is freely available at https://swissmodel.expasy.org.
同源建模已经成熟为结构生物学中的一项重要技术，极大地缩小了已知蛋白质序列和实验确定结构之间的差距。全自动工作流程和服务器简化和优化了同源建模过程，也允许没有特定计算专业知识的用户生成可靠的蛋白质模型，并轻松访问建模结果、可视化和解释。在这里，我们介绍了 SWISS-MODEL 服务器的更新，该服务器在 25 年前开创了自动化建模领域，并不断得到进一步发展。最近，其功能已扩展到同源和异源复合物的建模。从相互作用蛋白质的氨基酸序列开始，通过同源建模推断复合物的化学计量和整体结构。其他主要改进包括实现新的建模引擎 ProMod3 和引入新的局部模型质量估计方法 QMEANDisCo。SWISS-MODEL 可在 https://swissmodel.expasy.org 免费获得。
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