神经干细胞的单细胞转录组分析
Single-Cell Transcriptome Analysis of Neural Stem Cells.
作者信息
Li Ying, Anderson Jeremy, Kwan Kelvin Y, Cai Li
机构信息
Department of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, USA.
Department of Cell Biology & Neuroscience, Keck Center for Collaborative Research and Stem Cell Research Center, Rutgers University, 604 Allison Rd, Piscataway, NJ 08854, USA.
出版信息
Curr Pharmacol Rep. 2017 Apr;3(2):68-76. doi: 10.1007/s40495-017-0084-3. Epub 2017 Feb 27.
Neural stem cells (NSCs) in the adult central nervous system play essential roles in both normal homeostasis and repair of damaged tissue after injury. The study of adult NSCs is hampered by the heterogeneous NSC population. In this review, we describe recent progresses in using single-cell RNA-sequencing (scRNA-seq) technique for the investigation of NSCs. The first part of this review focuses on the scRNA-seq techniques and bioinformatic analysis. The second part emphasizes the applications of scRNA-seq analysis in NSC research. Finally, we discuss the challenges and future directions of scRNA-seq technique for both basic research and regenerative medicine.
成体中枢神经系统中的神经干细胞（NSCs）在正常内环境稳态以及损伤后受损组织的修复中均发挥着重要作用。成体神经干细胞群体的异质性阻碍了对其的研究。在本综述中，我们描述了使用单细胞RNA测序（scRNA-seq）技术研究神经干细胞的最新进展。本综述的第一部分聚焦于scRNA-seq技术和生物信息学分析。第二部分强调scRNA-seq分析在神经干细胞研究中的应用。最后，我们讨论了scRNA-seq技术在基础研究和再生医学方面面临的挑战及未来方向。
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