海洋来源菌株中 VioA 变体的异源表达激活了隐匿化合物。
Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Strain.
机构信息
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
Research Center for Marine Biology and Carbon Sequestration, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Biomass Energy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266003, China.
出版信息
Mar Drugs. 2018 Jun 2;16(6):191. doi: 10.3390/md16060191.
A new 14-membered homodimeric macrodiolide, brevidiolide (), along with four known aromatic compounds (, , and ) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived sp. 7002-073. The structures of ⁻ were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, showed notable growth inhibition against CCARM 3090 and ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL.
一株海洋来源的 sp. 7002-073 经表达 VioA G231L 变体的重组质粒 pWLI823 异源表达后，得到了一个新的 14 元同二聚大环二内酯 brevidiolide ()，以及四个已知的芳香族化合物（、、和）。通过 LC-MS 和 2D NMR 谱分析确定了 ⁻ 的结构。在评估化合物对多药耐药（MDR）菌株的抗菌活性时，显示出对 CCARM 3090 和 ATCC 13883 的显著生长抑制作用，最小抑菌浓度（MIC）值为 3.12 µg/mL。
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