丝状伴侣蛋白γ-前折叠蛋白向特定纳米结构的可控组装。

Controlled Assembly of the Filamentous Chaperone Gamma-Prefoldin into Defined Nanostructures.

作者信息

Clark Douglas S, Glover Dominic J

机构信息

Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.

School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia.

出版信息

Methods Mol Biol. 2018;1798:293-306. doi: 10.1007/978-1-4939-7893-9_22.

DOI:10.1007/978-1-4939-7893-9_22

PMID:29868968

Abstract

Self-assembling protein templates have enormous potential for the fabrication of multifunctional nanostructures that require precise positioning of individual molecules, such as enzymes and inorganic moieties, in regular patterns. A recently described approach uses ultrastable filaments composed of the gamma-prefoldin (γPFD) protein and engineered connector proteins to construct novel architectures useful for basic research and practical applications in nanobiotechnology. Here we describe the production of the γPFD and connector proteins from E. coli, and the assembly of γPFD with connector proteins into macromolecular structures with defined shapes.

摘要

自组装蛋白质模板在制造多功能纳米结构方面具有巨大潜力，这些纳米结构需要将单个分子（如酶和无机部分）以规则模式精确排列。最近描述的一种方法是使用由γ-前折叠蛋白（γPFD）和工程连接蛋白组成的超稳定细丝来构建对纳米生物技术的基础研究和实际应用有用的新型结构。在这里，我们描述了从大肠杆菌中生产γPFD和连接蛋白，以及将γPFD与连接蛋白组装成具有确定形状的大分子结构的过程。