鉴定急性髓细胞性白血病的 DNA 甲基化预后特征。
Identification of DNA methylation prognostic signature of acute myelocytic leukemia.
机构信息
Department of Hematology, Qilu Hospital, Shandong University, Jinan, Shandong, P.R. China.
Department of Hematology, Jining NO.1 People's Hospital, Jining, Shandong, P.R. China.
出版信息
PLoS One. 2018 Jun 22;13(6):e0199689. doi: 10.1371/journal.pone.0199689. eCollection 2018.
BACKGROUND
The aim of this study is to find the potential survival related DNA methylation signature capable of predicting survival time for acute myelocytic leukemia (AML) patients.
METHODS
DNA methylation data were downloaded. DNA methylation signature was identified in the training group, and subsequently validated in an independent validation group. The overall survival of DNA methylation signature was performed. Functional analysis was used to explore the function of corresponding genes of DNA methylation signature. Differentially methylated sites and CpG islands were also identified in poor-risk group.
RESULTS
A DNA methylation signature involving 8 DNA methylation sites and 6 genes were identified. Functional analysis showed that protein binding and cytoplasm were the only two enriched Gene Ontology terms. A total of 70 differentially methylated sites and 6 differentially methylated CpG islands were identified in poor-risk group.
CONCLUSIONS
The identified survival related DNA methylation signature adds to the prognostic value of AML.
背景
本研究旨在寻找潜在的与生存相关的 DNA 甲基化特征，以预测急性髓细胞白血病（AML）患者的生存时间。
方法
下载 DNA 甲基化数据。在训练组中确定 DNA 甲基化特征，然后在独立的验证组中进行验证。对 DNA 甲基化特征的总体生存率进行分析。功能分析用于探索 DNA 甲基化特征对应基因的功能。还在高危组中鉴定了差异甲基化位点和 CpG 岛。
结果
确定了一个涉及 8 个 DNA 甲基化位点和 6 个基因的 DNA 甲基化特征。功能分析表明，蛋白质结合和细胞质是仅有的两个富集的基因本体论术语。在高危组中总共鉴定出 70 个差异甲基化位点和 6 个差异甲基化 CpG 岛。
结论
所确定的与生存相关的 DNA 甲基化特征增加了 AML 的预后价值。
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