通过同源重组将DNA序列插入人类染色体β-珠蛋白基因座。
Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
作者信息
Smithies O, Gregg R G, Boggs S S, Koralewski M A, Kucherlapati R S
出版信息
Nature. 1985;317(6034):230-4. doi: 10.1038/317230a0.
DOI:10.1038/317230a0
PMID:2995814
Abstract
A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
摘要
一个含有珠蛋白基因序列、可与同源染色体序列发生重组的“可挽救”质粒，使我们能够产生、筛选并克隆出质粒整合到人β-珠蛋白基因座的哺乳动物细胞。无论靶基因是否表达，每千个转化细胞中约有一个实现了预期的修饰。
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