Dybowski Michal P, Dawidowicz Andrzej L
Faculty of Chemistry, Maria Curie Sklodowska University, Pl. Marii Curie Sklodowskiej 3, 20-031 Lublin, Poland.
Forensic Toxicol. 2018;36(2):415-423. doi: 10.1007/s11419-018-0419-8. Epub 2018 May 7.
Analysis of drugs and their metabolites in biofluids usually demands the application of sample preparation methods that allow for full isolation of analyzed substances from the matrix. The purpose of this study was to develop a method using the QuEChERS procedure for analysis of Δ-tetrahydrocannabinol (THC), 11-hydroxy-Δ-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Δ-tetrahydrocannabinol (11-COOH-THC).
THC, 11-OH-THC and 11-COOH-THC were quantified in whole blood samples using QuEChERS extraction and gas chromatography-tandem mass spectrometry.
The described method is characterized by good linearity, very low detection limits and satisfactory inter- and intraday precisions for THC, 11-OH-THC and 11-COOH-THC. The applicability of the procedure was confirmed using authentic whole blood samples collected from 30 persons suspected of driving under the influence of drugs.
The application of QuEChERS extraction described herein is a simple and convenient method for the routine analysis of THC, 11-OH-THC and 11-COOH-THC in whole blood samples from living and deceased humans. To our knowledge, this paper is the first academic report describing the QuEChERS extraction of THC and its metabolites from whole blood specimens.
对生物流体中的药物及其代谢物进行分析通常需要应用样品制备方法，以实现被分析物质与基质的完全分离。本研究的目的是开发一种使用QuEChERS方法分析Δ-四氢大麻酚（THC）、11-羟基-Δ-四氢大麻酚（11-OH-THC）和11-去甲-9-羧基-Δ-四氢大麻酚（11-COOH-THC）的方法。
采用QuEChERS萃取法和气相色谱-串联质谱法对全血样本中的THC、11-OH-THC和11-COOH-THC进行定量分析。
所描述的方法具有良好的线性、极低的检测限以及令人满意的THC、11-OH-THC和11-COOH-THC日内和日间精密度。通过对30名涉嫌药物影响下驾驶的人员采集的真实全血样本进行检测，证实了该方法的适用性。
本文所述的QuEChERS萃取法是一种用于常规分析活体和死亡人体全血样本中THC、11-OH-THC和11-COOH-THC的简单便捷方法。据我们所知，本文是第一篇描述从全血标本中采用QuEChERS萃取法提取THC及其代谢物的学术报告。
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