Genetic Analysis with Random Amplified Polymorphic DNA of the Multiple Enterocin-Producing 4CP3 Strain and Its Efficient Role in the Growth of in Raw Beef Meat.

In this manuscript, a multiple enterocin-producing strain named 4CP3 was used to control the proliferation of in refrigerated raw beef meat model. Also, the intraspecific genetic differentiation of 4CP3 strain was assessed by Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) analysis. 4CP3 strain was found to produce the enterocins A, B, and P. It displayed activity against EGDe 107776 by agar-well diffusion method. The application of 4CP3 culture at 10 CFU/g in raw beef meat was evaluated using both ANOVA and ANCOVA linear models in order to examine its effect on the growth of the pathogen during refrigerated storage. Hence, a very interesting result in decreasing (<0.05) and suppressing the growth of in refrigerated raw beef meat was shown during 28 days of storage. In conclusion, 4CP3 strain might be useful for prevention of the proliferation and survival of in raw meat during refrigerated storage.