Uzbekov Rustem, Garanina Anastasiia, Bressac Christophe
Department of Microscopy, University of Tours, Tours 37032, France
Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow 119992, Russia.
Biol Open. 2018 Aug 15;7(8):bio036012. doi: 10.1242/bio.036012.
The centrosome is the organizing center of microtubules in the cell, the basis for the origin of cilia and flagella and a site for the concentration of a regulatory proteins multitude. The centrosome comprises two centrioles surrounded by pericentriolar material. Centrioles in the cells of different organisms can contain nine triplets, doublets or singlets of microtubules. Here, we show that in somatic cells of male wasp larvae , centrioles do not contain microtubules and are composed of nine electron-dense prongs, which together form a cogwheel structure. These microtubule-free centrioles can be the platform for procentriole formation and form microtubule-free cilia-like structures. In nymph and imago cells centrioles have a microtubule triplet structure. Our study describes how centriole structure differs in a development-stage-dependent and a cell-type-dependent manner. The discovery of a centriole without microtubules casts a new light on the centriole formation process and the evolution of this organelle.
中心体是细胞中微管的组织中心，是纤毛和鞭毛起源的基础，也是多种调节蛋白聚集的场所。中心体由两个被中心粒周围物质包围的中心粒组成。不同生物体细胞中的中心粒可包含九个微管三联体、二联体或单联体。在这里，我们表明，在雄性黄蜂幼虫的体细胞中，中心粒不含微管，由九个电子致密的尖头组成，这些尖头共同形成一个齿轮结构。这些无微管的中心粒可以作为原中心粒形成的平台，并形成无微管的纤毛样结构。在若虫和成虫细胞中，中心粒具有微管三联体结构。我们的研究描述了中心粒结构如何以发育阶段依赖性和细胞类型依赖性方式存在差异。无微管中心粒的发现为中心粒形成过程和该细胞器的进化带来了新的启示。
