长链非编码RNA DANCR促进食管癌细胞的增殖、迁移、侵袭及抗凋亡能力。
Long non-coding RNA DANCR promotes cell proliferation, migration, invasion and resistance to apoptosis in esophageal cancer.
作者信息
Shi Hui, Shi Jiahai, Zhang Yudong, Guan Chengqi, Zhu Jun, Wang Fei, Xu Mingming, Ju Qianqian, Fang Shu, Jiang Maorong
机构信息
Laboratory Animals Center, Nantong University, Nantong 226001, China.
Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong 226001, China.
出版信息
J Thorac Dis. 2018 May;10(5):2573-2582. doi: 10.21037/jtd.2018.04.109.
BACKGROUND
Long non-coding RNAs (lncRNAs) have important effects on the development and progression of multiple carcinomas. Our studies aimed to investigate the expression of lncRNA DANCR in esophageal squamous cell carcinoma (ESCC) tissues and paracancerous tissues, and to explore its effect on the cell biological characteristics of ESCC ECA109 cells.
METHODS
The expression of DANCR was detected by qRT-PCR in human ESCC tissues and paracancerous normal tissues in ESCC patients. Small interfering RNA (siRNA) was transfected to knock down the expression of DANCR and interference efficiency was analyzed by qRT-PCR in ECA109 cells. MTT, wound healing, Transwell, TUNEL and flow cytometry (FCM) assay was used to measure the influence of DANCR on proliferation, invasion, migration and apoptosis in ECA109 cells, respectively.
RESULTS
The expression of DANCR in ESCC tissues and ESCC cells was significantly higher compared with that in the adjacent normal tissues (P<0.05). Furthermore, cell proliferation, migration and invasion were significantly suppressed by knock-down mediated down-regulation of DANCR expression. On the contrary, cell apoptosis was promoted by silencing of DANCR.
CONCLUSIONS
According to our research, the expression of DANCR was up-regulated in human ESCC tissues, and the important role that DANCR played in ESCC cells was similar to an oncogene. Therefore, silencing of lncRNA DANCR could have potentially beneficial effects on the prognostic and therapy for ESCC in the future.
背景
长链非编码RNA（lncRNAs）对多种癌症的发生发展具有重要影响。我们的研究旨在探讨lncRNA DANCR在食管鳞状细胞癌（ESCC）组织和癌旁组织中的表达情况，并探究其对ESCC ECA109细胞生物学特性的影响。
方法
采用qRT-PCR检测ESCC患者的ESCC组织及癌旁正常组织中DANCR的表达。转染小干扰RNA（siRNA）敲低ECA109细胞中DANCR的表达，并通过qRT-PCR分析干扰效率。分别采用MTT、伤口愈合实验、Transwell实验、TUNEL实验和流式细胞术（FCM）检测DANCR对ECA109细胞增殖、侵袭、迁移和凋亡的影响。
结果
与相邻正常组织相比，ESCC组织和ESCC细胞中DANCR的表达显著升高（P<0.05）。此外，敲低介导的DANCR表达下调显著抑制了细胞增殖、迁移和侵袭。相反，沉默DANCR可促进细胞凋亡。
结论
根据我们的研究，DANCR在人ESCC组织中的表达上调，且DANCR在ESCC细胞中发挥的重要作用类似于癌基因。因此，沉默lncRNA DANCR可能对未来ESCC的预后和治疗具有潜在的有益作用。
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