Kim Min Jung, Lee Da-Hye, Ahn Jiyun, Ha Tae-Youl, Jang Young Jin, Do Eunju, Jung Chang Hwa
Division of Nutrition and Metabolism Research, Korea Food Research Institute, Seongnam, Republic of Korea.
Department of Food Biotechnology, Korea University of Science and Technology, Seongnam, Republic of Korea.
Evid Based Complement Alternat Med. 2018 May 30;2018:9214960. doi: 10.1155/2018/9214960. eCollection 2018.
Although classification of an individual's Sasang constitution is a key step in the prescription of traditional Korean medicine, the classifying process is complex and not objective. Identification of metabolic-based biomarkers could allow the development of a reliable and sensitive classification technique and even therapeutic management. Our pilot study investigated whether metabolites in plasma are characteristic of Sasang constitutions. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-based metabolic analysis was conducted against 15 Soyangin (SY), 15 Taeeumin (TE), and 18 Soeumin (SE) individuals, as classified according to the Questionnaire for Sasang Constitution Classification II (QSCC II) and specialist diagnosis. Metabolomics data showed that the TE group was significantly separated from the SY and SE groups. Nine canonical pathways related to constitution; phenylalanine metabolism, aminoacyl-tRNA, tyrosine, and tryptophan biosynthesis were activated in the TE group as compared with the other groups. Similar to the results of the metabolomics analysis, the TE group was also significantly separated from the other two groups by lipidomic analysis. On the other hand, the intensity of lipid metabolites was higher in the SY group than in the other groups. Our findings suggest that the combined analysis of metabolomics and lipidomics can provide useful information for characteristics of Sasang constitutions.
尽管个体的体质分类是韩医开方的关键步骤，但分类过程复杂且不客观。基于代谢的生物标志物的识别可以开发出可靠且灵敏的分类技术，甚至用于治疗管理。我们的初步研究调查了血浆中的代谢物是否具有体质特征。根据体质分类问卷II（QSCC II）和专家诊断，对15名少阳人（SY）、15名太阴人（TE）和18名少阴人（SE）进行了基于超高效液相色谱-四极杆飞行时间质谱的代谢分析。代谢组学数据显示，TE组与SY组和SE组有明显区分。与其他组相比，TE组中有9条与体质相关的典型途径被激活；苯丙氨酸代谢、氨酰-tRNA、酪氨酸和色氨酸生物合成。与代谢组学分析结果相似，脂质组学分析也显示TE组与其他两组有明显区分。另一方面，SY组中脂质代谢物的强度高于其他组。我们的研究结果表明，代谢组学和脂质组学的联合分析可以为体质特征提供有用信息。
