CD32 表达与 T 细胞激活相关，而不是 HIV-1 储存库的标志物。
CD32 expression is associated to T-cell activation and is not a marker of the HIV-1 reservoir.
机构信息
AIDS Research Institute-IrsiCaixa and Health Research Institute Germans Trias i Pujol (IGTP), Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, 08916, Badalona, Spain.
出版信息
Nat Commun. 2018 Jul 16;9(1):2739. doi: 10.1038/s41467-018-05157-w.
CD32 has been shown to be preferentially expressed in latently HIV-1-infected cells in an in vitro model of quiescent CD4 T cells. Here we show that stimulation of CD4+ T cells with IL-2, IL-7, PHA, and anti-CD3/CD28 antibodies induces T-cell proliferation, co-expression of CD32 and the activation of the markers HLA-DR and CD69. HIV-1 infection increases CD32 expression. 79.2% of the CD32+/CD4+ T cells from HIV+ individuals under antiretroviral treatment were HLA-DR+. Resting CD4+ T cells infected in vitro generally results in higher integration of provirus. We observe no difference in provirus integration or replication-competent inducible latent HIV-1 in CD32+ or CD32- CD4+ T cells from HIV+ individuals. Our results demonstrate that CD32 expression is a marker of CD4+ T cell activation in HIV+ individuals and raises questions regarding the immune resting status of CD32+ cells harboring HIV-1 proviruses.
CD32 在体外静止 CD4 T 细胞模型中优先表达潜伏 HIV-1 感染细胞。在这里，我们发现刺激 CD4+T 细胞的白细胞介素-2、白细胞介素-7、PHA 和抗 CD3/CD28 抗体可诱导 T 细胞增殖、CD32 共表达和 HLA-DR 和 CD69 标志物的激活。HIV-1 感染增加 CD32 的表达。在接受抗逆转录病毒治疗的 HIV+个体中，79.2%的 CD32+/CD4+T 细胞为 HLA-DR+。体外感染静止的 CD4+T 细胞通常会导致前病毒更高的整合。我们在 HIV+个体的 CD32+或 CD32-CD4+T 细胞中未观察到前病毒整合或复制活性诱导潜伏 HIV-1 的差异。我们的结果表明，CD32 表达是 HIV+个体中 CD4+T 细胞活化的标志物，并提出了关于携带 HIV-1 前病毒的 CD32+细胞免疫静止状态的问题。
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