采用 MALDI-MSI 技术对盐酸特比萘芬在活体皮肤等效模型中的吸收进行定量研究。
Quantitative Investigation of Terbinafine Hydrochloride Absorption into a Living Skin Equivalent Model by MALDI-MSI.
机构信息
Centre for Mass Spectrometry Imaging, Biomolecular Research Centre , Sheffield Hallam University , Howard Street , Sheffield S1 1WB , U.K.
Croda International Plc , Cowick Hall , Snaith , Goole, East Yorkshire DN14 9AA , U.K.
出版信息
Anal Chem. 2018 Aug 21;90(16):10031-10038. doi: 10.1021/acs.analchem.8b02648. Epub 2018 Aug 2.
The combination of microspotting of analytical and internal standards, matrix sublimation, and recently developed software for quantitative mass spectrometry imaging has been used to develop a high-resolution method for the determination of terbinafine hydrochloride in the epidermal region of a full thickness living skin equivalent model. A quantitative assessment of the effect of the addition of the penetration enhancer (dimethyl isosorbide (DMI)) to the delivery vehicle has also been performed, and data have been compared to those obtained from LC-MS/MS measurements of homogenates of isolated epidermal tissue. At 10% DMI, the levels of signal detected for the drug in the epidermis were 0.20 ± 0.072 mg/g tissue for QMSI and 0.28 ± 0.040 mg/g tissue for LC-MS/MS at 50% DMI 0.69 ± 0.23 mg/g tissue for QMSI and 0.66 ± 0.057 mg/g tissue for LC-MS/MS. Comparison of means and standard deviations indicates no significant difference between the values obtained by the two methods.
微点分析和内标物的组合、基质升华以及最近开发的定量质谱成像软件，已经被用于开发一种高分辨率方法，以测定全厚度活体皮肤等效模型表皮区域中的盐酸特比萘芬含量。还对添加渗透增强剂（二甲基异山梨醇（DMI））对给药载体的影响进行了定量评估，并将数据与从分离的表皮组织匀浆中进行 LC-MS/MS 测量获得的数据进行了比较。在 10% DMI 时，QMSI 检测到的药物在表皮中的信号水平为 0.20 ± 0.072 mg/g 组织，LC-MS/MS 为 0.28 ± 0.040 mg/g 组织；在 50% DMI 时，QMSI 检测到的信号水平为 0.69 ± 0.23 mg/g 组织，LC-MS/MS 为 0.66 ± 0.057 mg/g 组织。均值和标准差的比较表明，两种方法获得的值之间没有显著差异。
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