研究四氯二苯并对二噁英诱导的聚ADP核糖聚合酶（TIPARP）单ADP核糖基转移酶活性的方法。
Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity.
作者信息
Hutin David, Grimaldi Giulia, Matthews Jason
机构信息
Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
出版信息
Methods Mol Biol. 2018;1813:109-124. doi: 10.1007/978-1-4939-8588-3_8.
TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP-ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP's catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.
四氯二苯并对二恶英诱导的多聚 ADP 核糖聚合酶（TIPARP；也称为 PARP7 和 ARTD14）是一种单 ADP 核糖基转移酶，已成为先天性免疫、干细胞多能性和转录因子调控的重要调节因子。表征 TIPARP 的催化活性并鉴定其靶蛋白对于理解其细胞功能至关重要。在这里，我们描述了用于表征 TIPARP 催化活性及其对靶蛋白的单 ADP 核糖基化作用的方法。
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