Akyavuz Rıfat, Taskin Bilgin, Koçak Metin, Yildiz Mehtap
Department of Agricultural Biotechnology, Faculty of Agriculture, Van Yuzuncu Yil University, Van, Turkey.
3 Biotech. 2018 Aug;8(8):355. doi: 10.1007/s13205-018-1380-2. Epub 2018 Aug 2.
is thought as one of the most diverse and significant genera due to its varied uses in different parts of the world. In this study, we worked with a total of 71 pepper genotypes from different locations of Turkey to investigate the level of their diversity using the peroxidase gene polymorphism (POGP) markers to reveal their population structure. For this purpose, 14 peroxidase primer pairs were used. They produced 139 bands (mean = 9.9 bands/primer), of which ~ 85.6% were polymorphic in the all germplasm collection. Polymorphism information content (PIC) ranged between 0.48 and 0.97 with an average of 0.75. Range and mean values for gene diversity () were 0.09-0.22 and 0.17, respectively. Shannon's information index () per POGP marker ranged from 0.18 to 0.35 with a mean of 0.29. Using three clustering methods (unweighted pair-group method with arithmetic means, principal coordinate analysis, and STRUCTURE) revealed a clear separation of all the accessions from and accessions in our study. Clusters did not establish an association between the accessions and their geographical origin. This is the first study exploring the population structure through the genetic diversity of Turkish peppers from different regions of the country based on the peroxidase gene markers.
由于其在世界不同地区的多种用途,被认为是最多样化和最重要的属之一。在本研究中,我们使用来自土耳其不同地点的总共71种辣椒基因型,通过过氧化物酶基因多态性(POGP)标记来研究它们的多样性水平,以揭示其群体结构。为此,使用了14对过氧化物酶引物。它们产生了139条带(平均每条引物产生9.9条带),其中在所有种质资源中约85.6%是多态性的。多态性信息含量(PIC)在0.48至0.97之间,平均为0.75。基因多样性()的范围和平均值分别为0.09 - 0.22和0.17。每个POGP标记的香农信息指数()范围为0.18至0.35,平均值为0.29。使用三种聚类方法(算术平均数的非加权配对组法、主坐标分析和STRUCTURE)揭示了在我们的研究中所有种质与种质之间的明显分离。聚类并未在种质与其地理起源之间建立关联。这是第一项基于过氧化物酶基因标记,通过来自该国不同地区的土耳其辣椒的遗传多样性来探索群体结构的研究。