载 BMP-2 的 3D 多孔磺化聚醚醚酮（PEEK）的可控且持久释放，用于增强成骨活性。
Controllable and durable release of BMP-2-loaded 3D porous sulfonated polyetheretherketone (PEEK) for osteogenic activity enhancement.
机构信息
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, China; The Key Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of the Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai 200234, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, China; University of Chinese Academy of Science, Beijing 100049, China.
出版信息
Colloids Surf B Biointerfaces. 2018 Nov 1;171:668-674. doi: 10.1016/j.colsurfb.2018.08.012. Epub 2018 Aug 8.
Polyetheretherketone (PEEK) is ideal for dental and orthopedic applications because its mechanical properties are similar to cortical bones. However, its inherent inert ability hinders its clinical applications. In this work, bone morphogenetic protein-2 (BMP-2) was immobilized onto the sulfonated PEEK (SPEEK) using lyophilization technology. The surface morphologies of the samples were analyzed by field-emission scanning electron microscopy (FE-SEM), and the chemical compositions were analyzed by energy-dispersive X-ray spectrometry (EDS). The release content of BMP-2 of the samples immersed in the PBS (pH = 7.4) was detected by a human BMP-2 ELISA kit. The results indicated that controllable and durable BMP-2 release was accomplished due to the three-dimensional (3D) network of sulfonated PEEK. The in vitro cellular experiments showed that the BMP-2-immobilized samples significantly enhanced the initial adhesion and spreading of rat bone mesenchymal stem cells (rBMSCs). Moreover, the collagen secretion, extracellular matrix mineralization and ALP activity were also improved. Thus, the BMP-2-immobilized samples greatly promoted the osteogenic differentiation of rBMSCs, which revealed that BMP-2 immobilization paves the way for the use of PEEK in clinical applications.
聚醚醚酮（PEEK）因其机械性能类似于皮质骨，是牙科和骨科应用的理想选择。然而，其固有的惰性能力阻碍了其临床应用。在这项工作中，骨形态发生蛋白-2（BMP-2）通过冷冻干燥技术固定在磺化聚醚醚酮（SPEEK）上。通过场发射扫描电子显微镜（FE-SEM）分析样品的表面形态，通过能谱仪（EDS）分析化学组成。通过人 BMP-2 ELISA 试剂盒检测样品在 PBS（pH=7.4）中浸提的 BMP-2 释放含量。结果表明，由于磺化 PEEK 的三维（3D）网络，实现了可控且持久的 BMP-2 释放。体外细胞实验表明，固定 BMP-2 的样品显著增强了大鼠骨髓间充质干细胞（rBMSCs）的初始粘附和铺展。此外，还改善了胶原蛋白分泌、细胞外基质矿化和碱性磷酸酶活性。因此，固定 BMP-2 的样品大大促进了 rBMSCs 的成骨分化，这表明 BMP-2 固定为 PEEK 在临床应用中的使用铺平了道路。
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