Tian Yujing, Zhang Jiukai, Cheng Haiyan, Li Xian, Chen Ying
Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Department of Horticulture, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou 310058, China.
Se Pu. 2018 Jul 8;36(7):588-598. doi: 10.3724/SP.J.1123.2018.01016.
Proteomics, as a new research direction in the post-genomics era, has been developing rapidly in recent years and has been applied in many fields, becoming a powerful research tool in food quality identification and safety control. Proteomics opens up a new horizon in food science research. It can not only identify the species of characteristic proteins, but also quantify the response of targeted proteins. Proteomics allows for the dynamic analysis of protein composition and content in samples that vary in species, geographical origin or growth stage. The research methods of proteomics are varied, and mass spectrometry (MS) is one of the most common techniques. This paper introduces the concept, classification, and research technology of proteomics, as well as common protein databases. Applications of MS-based proteomics in food authentication and quality identification were reviewed, including seafood, meat products, dairy products, health food, and value-added food. Furthermore, the future development of proteomics was investigated.
蛋白质组学作为后基因组时代的一个新的研究方向,近年来发展迅速,并已应用于许多领域,成为食品质量鉴定和安全控制中的一种强大研究工具。蛋白质组学为食品科学研究开辟了新视野。它不仅可以鉴定特征蛋白的种类,还可以对目标蛋白的响应进行定量。蛋白质组学能够对物种、地理来源或生长阶段不同的样品中的蛋白质组成和含量进行动态分析。蛋白质组学的研究方法多种多样,质谱(MS)是最常用的技术之一。本文介绍了蛋白质组学的概念、分类、研究技术以及常见的蛋白质数据库。综述了基于质谱的蛋白质组学在食品认证和质量鉴定中的应用,包括海鲜、肉类产品、乳制品、保健食品和增值食品。此外,还探讨了蛋白质组学的未来发展。