一种用于超灵敏检测癌胚抗原的新型磁弹性免疫传感器。
A Novel Magnetoelastic Immunosensor for Ultrasensitively Detecting Carcinoembryonic Antigen.
作者信息
Zhang Yixia, Guo Xing, Fan Lu, Zhang Qiang, Sang Shengbo
机构信息
Institute of Applied Mechanics and Biomedical Engineering & National Demonstration Center for Experimental Mechanics Education, College of Mechanics, Taiyuan University of Technology, Taiyuan, 030024, China.
Micro-Nano System Research Center & Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
出版信息
Nanoscale Res Lett. 2018 Aug 29;13(1):258. doi: 10.1186/s11671-018-2632-0.
A novel wireless immunosensor is developed for the ultra-sensitive detection of carcinoembryonic antigen. The optimum dimension of the microchips, as magnetoelastic sensitive units, was evaluated by simulation and experiments. The unique effects signal amplification and biocompatibility of gold particles contribute to the stability and sensitivity of the sensor. Furthermore, to enhance sensitivity, the working concentrations of antibody and BSA are selected to be 50 mg/mL and 0.1%, respectively. Atom force microscope imaging sheds light on the biological analysis. The Nano-magnetoelastic immunosensor exhibits a linear response to the logarithm of carcinoembryonic antigen (CEA) concentrations ranging from 0.1 to 100 ng/mL, with a detection limit of 2.5 pg/mL. The designed biosensor has merits of excellent stability and sensitivity towards CEA.
一种用于超灵敏检测癌胚抗原的新型无线免疫传感器被开发出来。作为磁弹性敏感单元的微芯片的最佳尺寸通过模拟和实验进行了评估。金颗粒独特的信号放大和生物相容性效应有助于提高传感器的稳定性和灵敏度。此外，为了提高灵敏度，抗体和牛血清白蛋白的工作浓度分别选择为50 mg/mL和0.1%。原子力显微镜成像有助于生物分析。纳米磁弹性免疫传感器对癌胚抗原（CEA）浓度在0.1至100 ng/mL范围内的对数呈现线性响应，检测限为2.5 pg/mL。所设计的生物传感器对CEA具有出色的稳定性和灵敏度。
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