建立来自共济失调毛细血管扩张症和色素性干皮病患者且具有高辐射敏感性的细胞系。

Establishment of cell lines derived from ataxia telangiectasia and xeroderma pigmentosum patients with high radiation sensitivity.

作者信息

Hashimoto T, Nakano Y, Owada M K, Kakunaga T, Furuyama J

出版信息

Mutat Res. 1986 Sep;166(2):215-20. doi: 10.1016/0167-8817(86)90020-9.

PMID:3020400

Abstract

Four human fibroblast cell lines, three of which were derived from a patient with ataxia telangiectasia and the other from a patient with xeroderma pigmentosum, were established after transfection with cloned SV40 DNA. These 4 cell lines showed some phenotypes characteristic of neoplastically transformed cells, and had a human karyotype with heteromorphisms identical to those of the parental fibroblasts. Their sensitivity to the cytotoxic effects of gamma-rays or ultraviolet irradiation was as high as those of their parental fibroblasts.

摘要

用克隆的SV40 DNA转染后建立了4个人成纤维细胞系，其中3个源自共济失调毛细血管扩张症患者，另1个源自着色性干皮病患者。这4个细胞系表现出一些肿瘤转化细胞的特征性表型，并且具有与亲代成纤维细胞相同的人类核型异态性。它们对γ射线或紫外线照射的细胞毒性作用的敏感性与亲代成纤维细胞一样高。