兔滑膜细胞胶原酶基因组克隆中含外显子部分与人类包皮及滑膜细胞信使核糖核酸之间的同源性。
Homology between exon-containing portions of rabbit genomic clones for synovial cell collagenase and human foreskin and synovial cell mRNAs.
作者信息
Fini M E, Austin S D, Holt P T, Ruby P L, Gross R H, White H D, Brinckerhoff C E
出版信息
Coll Relat Res. 1986 Jul;6(3):239-48. doi: 10.1016/s0174-173x(86)80009-7.
Portions of rabbit genomic clones containing exons cross-hybridize with human synovial cell and human foreskin fibroblast mRNAs. These cross-hybridizing genomic fragments have been subcloned into pBR328 and may be useful probes in isolating cDNA and genomic clones from human tissue, and perhaps from other species as well. In addition, DNA sequence data on a 530 bp cDNA clone for rabbit synovial-cell collagenase indicate that this clone is composed primarily of 3'-untranslated region. As such, it is probably not useful in cross-species hybridizations.
含有外显子的兔基因组克隆片段与人类滑膜细胞和人类包皮成纤维细胞的信使核糖核酸发生交叉杂交。这些发生交叉杂交的基因组片段已被亚克隆到pBR328中，可能是从人类组织甚至其他物种中分离互补脱氧核糖核酸和基因组克隆的有用探针。此外，关于兔滑膜细胞胶原酶的一个530碱基对互补脱氧核糖核酸克隆的脱氧核糖核酸序列数据表明，该克隆主要由3'-非翻译区组成。因此，它可能在跨物种杂交中无用。
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