来自巴西甜瓜植株的葫芦科蚜传黄化病毒是一种种间重组体。
Cucurbit aphid-borne yellows virus from melon plants in Brazil is an interspecific recombinant.
作者信息
Costa Thiago Marques, Blawid Rosana, Aranda Miguel A, Freitas Débora Maria Sansini, Andrade Genira Pereira, Inoue-Nagata Alice Kazuko, Nagata Tatsuya
机构信息
Campus Darcy Ribeiro, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70910-900, Brazil.
Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), 30100, Murcia, Spain.
出版信息
Arch Virol. 2019 Jan;164(1):249-254. doi: 10.1007/s00705-018-4024-2. Epub 2018 Sep 19.
Melon plants with severe yellowing symptoms from in Brazil were analyzed by high-throughput sequencing. Sequences homologous to the genome of the polerovirus cucurbit aphid-borne yellows virus (CABYV) were frequently retrieved. Two draft CABYV genomes were assembled from two pooled melon samples that contained an identical putative recombinant fragment in the 3' region with an unknown polerovirus. The complete genomes of these isolates revealed by Sanger sequencing share 96.8% nucleotide identity, while both sequences share 73.7% nucleotide identity with a CABYV-N isolate from France. A molecular-clock analysis suggested that CABYV was introduced into Brazil ~ 68 years ago.
对巴西出现严重黄化症状的甜瓜植株进行了高通量测序分析。经常检索到与马铃薯Y病毒组的葫芦蚜传黄化病毒（CABYV）基因组同源的序列。从两个混合的甜瓜样本中组装了两个CABYV基因组草图，这两个样本在3'区域含有一个与未知马铃薯Y病毒相同的假定重组片段。通过桑格测序揭示的这些分离株的完整基因组具有96.8%的核苷酸同一性，而这两个序列与来自法国的CABYV-N分离株均具有73.7%的核苷酸同一性。分子钟分析表明，CABYV大约在68年前被引入巴西。
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