微波照射与化学和机械方法对被……污染的全口义齿消毒效果的比较
comparison of the effects of microwave irradiation and chemical and mechanical methods on the disinfection of complete dentures contaminated with .
作者信息
Aslanimehr Masoomeh, Mojarad Niloofar, Ranjbar Safarali, Aalaei Shima
机构信息
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
出版信息
Dent Res J (Isfahan). 2018 Sep-Oct;15(5):340-346.
BACKGROUND
Dentures can be a source of infection or cross-contamination with microorganisms like . The aim of this study was to compare the effects of microwave irradiation, chemical techniques, and a mechanical method (i.e., brushing) on the disinfection of complete dentures contaminated with .
MATERIALS AND METHODS
In this experimental study, sixty sterilized dentures were divided into six groups of 10 dentures each. The dentures in Groups 1 and 6 served as negative and positive controls, respectively. The dentures (Groups 2-5) were contaminated with and subjected to four disinfection procedures: Corega tablets, 2% glutaraldehyde, brushing, and microwave irradiation. Replicate aliquots (25 mL) of the suspension were cultured in Sabouraud dextrose broth. The colonies were counted after 48 h of incubation at 37°C. To confirm long-term disinfection, the Trypticase soy broth (TSB) containers were stored at 37°C for 7 days, and turbidity was visually observed. Data were analyzed with one-way ANOVA and independent-samples -test on SPSS the level of statistical significance was set at 0.05.
RESULTS
The dentures disinfected with microwave irradiation (650 W, 3 min) and glutaraldehyde (2%, 10 min) exhibited no evidence of fungal growth after 48 h of incubation and also no turbidity in the TSB containers after 7 days of incubation. However, the dentures disinfected using the mechanical method and Corega tablets exhibited turbidity after 7 days and fungal growth after 48 h that was significantly more than that in the two other methods ( = 0.000) and less than that in the positive control group ( = 0.000). The differences between mechanical cleaning and cleansing tablet were not significant ( = 0.017).
CONCLUSION
Base on the results of this study, microwave irradiation (650 W, 3 min) and 2% glutaraldehyde completely disinfected the dentures contaminated with in the short term and long term.
背景
假牙可能是感染源或与诸如……等微生物发生交叉污染的源头。本研究的目的是比较微波照射、化学技术和机械方法（即刷牙）对被……污染的全口假牙消毒的效果。
材料与方法
在本实验研究中，60副灭菌假牙被分为6组，每组10副。第1组和第6组的假牙分别作为阴性和阳性对照。第2 - 5组的假牙被……污染，并接受四种消毒程序：使用可力康片剂、2%戊二醛、刷牙和微波照射。将25毫升悬浮液的重复等分试样接种于沙氏葡萄糖肉汤中培养。在37℃孵育48小时后计数菌落。为确认长期消毒效果，将胰蛋白胨大豆肉汤（TSB）容器在37℃下储存7天，并肉眼观察浑浊情况。数据在SPSS上用单因素方差分析和独立样本t检验进行分析，统计学显著性水平设定为0.05。
结果
经微波照射（650瓦，3分钟）和戊二醛（2%，10分钟）消毒的假牙在孵育48小时后未显示真菌生长迹象，在孵育7天后TSB容器中也无浑浊现象。然而，使用机械方法和可力康片剂消毒的假牙在7天后出现浑浊，在48小时后出现真菌生长，且明显多于其他两种方法（P = 0.000），并少于阳性对照组（P = 0.000）。机械清洁和清洁片剂之间的差异不显著（P = 0.017）。
结论
基于本研究结果，微波照射（650瓦，3分钟）和2%戊二醛在短期和长期内都能完全消毒被……污染的假牙。
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