橙皮素对γ射线诱导的小鼠脾细胞DNA损伤和免疫功能障碍的辐射防护作用。
Radioprotective effect of hesperetin against γ-irradiation-induced DNA damage and immune dysfunction in murine splenocytes.
作者信息
Kang Jung Ae, Yoon Seon Hye, Rho Jong Kook, Jang Beom-Su, Choi Dae Seong, Lee Dong-Eun, Byun Eui-Baek, Jeon Jongho, Park Sang Hyun
机构信息
1Division of Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 56212 Korea.
2Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation, Osong, Chungbuk, 28159 Korea.
出版信息
Food Sci Biotechnol. 2016 Mar 31;25(Suppl 1):163-168. doi: 10.1007/s10068-016-0114-7. eCollection 2016.
This study was conducted to evaluate the preventive effect of hesperetin against radiation-induced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 µM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to ?-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.
本研究旨在评估橙皮素对小鼠脾细胞辐射诱导的DNA损伤和免疫功能障碍的预防作用。从BALB/c小鼠分离出的脾细胞用橙皮素（20、100和500 μM）处理，然后以2 Gy和4 Gy的剂量进行γ射线照射。暴露于γ射线导致DNA损伤、细胞活力降低以及促炎细胞因子、细胞内活性氧（ROS）和一氧化氮（NO）水平升高。与照射组相比，橙皮素显著提高了脾细胞的活力。此外，通过彗星试验和DNA梯状条带分析发现，橙皮素在预防DNA损伤方面非常有效。橙皮素还能有效抑制照射后脾细胞中的促炎细胞因子、细胞内ROS和NO。总之，橙皮素对小鼠脾细胞辐射诱导的DNA损伤和免疫功能障碍具有辐射防护作用。
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