通过聚多巴胺将琼脂酶固定在磁性 ferriferous 上的生物粘附。
Bioadhesive immobilize agarase on magnetic ferriferous by polydopamine.
机构信息
College of Chemical Engineering & Materials Science, Tianjin University of Science & Technology, Tianjin 300457, PR China; Tianjin Key Laboratory of Marine Resources & Chemistry, Tianjin University of Science & Technology, Tianjin 300457, PR China.
College of Chemical Engineering & Materials Science, Tianjin University of Science & Technology, Tianjin 300457, PR China; Tianjin Key Laboratory of Marine Resources & Chemistry, Tianjin University of Science & Technology, Tianjin 300457, PR China.
出版信息
Mater Sci Eng C Mater Biol Appl. 2018 Dec 1;93:218-225. doi: 10.1016/j.msec.2018.07.068. Epub 2018 Jul 24.
The combined action of dopamine-iron coordination reaction and dopamine self-polymerization was employed to immobilize agarase on the surface of Magnetic ferriferous oxide (MFO). The solid-liquid separation was easily realized in magnetic field due to the magnetic carrier. The immobilization process was taken by dopamine self-polymerization in room temperature, aqueous solution and neutral pH without organic solvent. Magnetic ferriferous oxide was prepared through coprecipitation. Then, MFO and dopamine were reacted under mild conditions to prepare the magnetic ferriferous oxide-polydopamine (MFO-PDA) complex. The MFO-PDA complex was used in enzyme immobilization. It showed that the average enzyme activity yield was 63.9% in Immobilized enzyme, and the relative enzyme activity of the immobilized enzyme was still above 50% even after five times of reuse in addition, the MFO-PDA complex had good immobilization capacity toward agarase, and the immobilized enzyme has good reusability.
采用多巴胺-铁配位反应和多巴胺自聚合的协同作用将琼脂酶固定在磁性四氧化三铁（MFO）表面。由于磁性载体，很容易在磁场中实现固液分离。固定化过程在室温、水溶液和中性 pH 值下进行，无需有机溶剂。通过共沉淀法制备磁性四氧化三铁。然后，在温和条件下使 MFO 和多巴胺反应，制备磁性四氧化三铁-聚多巴胺（MFO-PDA）复合物。MFO-PDA 复合物用于酶固定化。结果表明，固定化酶的平均酶活收率为 63.9%，固定化酶的相对酶活在重复使用五次后仍保持在 50%以上。此外，MFO-PDA 复合物对琼脂酶具有良好的固定化能力，固定化酶具有良好的可重复使用性。
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