BIL1/BZR1 识别油菜素内酯的结构基础。

Structural basis for brassinosteroid response by BIL1/BZR1.

机构信息

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Department of Biochemistry, King Abdulaziz University, Jeddah, Saudi Arabia.

出版信息

Nat Plants. 2018 Oct;4(10):771-776. doi: 10.1038/s41477-018-0255-1. Epub 2018 Oct 1.

DOI:10.1038/s41477-018-0255-1

PMID:30287951

Abstract

BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) is a master transcription factor of brassinosteroid (BR) signalling. The varieties of nucleobase recognition of the NN-BRRE-core motif (NNCGTG), one of variant G-box motifs, distinguish BIL1/BZR1 from basic helix-loop-helix transcription factors, underlying the specific regulation of BR-responsive genes. Here, we show the non-canonical bHLH dimer formation of BIL1/BZR1 to optimize the interaction network with DNA and the orientation of a key residue for NN-BRRE-core motif recognition.

摘要

BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) 是油菜素内酯 (BR) 信号转导的主转录因子。NN-BRRE-core 基序（NNCGTG）的核碱基识别变体 G 盒基序的多样性，将 BIL1/BZR1 与碱性螺旋-环-螺旋转录因子区分开来，从而对 BR 响应基因进行特异性调控。在这里，我们展示了 BIL1/BZR1 的非典型 bHLH 二聚体形成，以优化与 DNA 的相互作用网络和关键残基的方向，用于 NN-BRRE-core 基序识别。