通过改良的亲水聚二甲基硅氧烷微阵列芯片制备具有可控尺寸的巨型脂质体。
Preparation of giant lipid vesicles with controllable sizes by a modified hydrophilic polydimethylsiloxane microarray chip.
机构信息
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China; Chongqing Engineering Research Center of Medical Electronics Technology (Chongqing University), Chongqing University, Chongqing 400030, China.
出版信息
J Colloid Interface Sci. 2019 Feb 15;536:53-61. doi: 10.1016/j.jcis.2018.10.034. Epub 2018 Oct 15.
This paper presents an accessible method to prepare giant lipid vesicles (GLVs) with controllable sizes based on the quick formation of patterned lipid films. Lipid solutions naturally penetrate into arrayed micro-apertures on a modified hydrophilic Polydimethylsiloxane (PDMS) chip, and excess lipid films on the surface are removed by a glass slide. Three main factors, the depth and diameter of the micro-apertures and concentration of the lipid solution, were investigated to obtain an optimal preparation condition. Based on this condition, the formed GLVs have a controllable size and narrow size distribution (the standard deviation < 5 μm). By controlling the diameter of the micro-aperture and concentration of the lipid solution, GLVs with various sizes (23, 48, 66 and 82 μm) can be formed.
本文提出了一种可制备具有可控尺寸的巨型脂质体（GLV）的方法，该方法基于图案化脂质膜的快速形成。脂质溶液自然地渗透到经过修饰的亲水聚二甲基硅氧烷（PDMS）芯片上的阵列微孔中，并用玻璃片去除表面多余的脂质膜。研究了三个主要因素，即微孔的深度和直径以及脂质溶液的浓度，以获得最佳的制备条件。在此条件下，形成的 GLV 具有可控的尺寸和较窄的尺寸分布（标准偏差<5μm）。通过控制微孔的直径和脂质溶液的浓度，可以形成各种尺寸（23、48、66 和 82μm）的 GLV。
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