血清 miR-139-5p 作为指标在骨肉瘤中的诊断效果。

The diagnostic effect of serum miR-139-5p as an indicator in osteosarcoma.

机构信息

Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.

State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China.

出版信息

Cancer Biomark. 2018;23(4):561-567. doi: 10.3233/CBM-181744.

DOI:10.3233/CBM-181744

PMID:30347602

Abstract

BACKGROUND

The microRNA, miR-139-5p, plays an important role in the initiation and progression of various tumor types including osteosarcoma (OS).

OBJECTIVE

This study aimed to detect the serum miR-139-5p expression in OS and analyze its association with clinical variables.

METHODS

Blood samples were taken from 98 OS patients and 50 healthy individuals, and serum miR-139-5p levels were measured by quantitative reverse transcription-polymerase chain reaction.

RESULTS

We found the expression of serum miR-139-5p in OS patients was significantly lower than that in healthy individuals, and miR-139-5p levels were dramatically decreased in patients with distant metastasis or in higher clinical stage. Receiver-operating characteristic (ROC) analysis revealed that serum miR-139-5p could well discriminate OS patients from healthy controls with a high sensitivity and specificity. Moreover, low serum miR-139-5p expression was strongly associated with distant metastasis, tumor stage and shorter overall survival. Both univariate and multivariate analyses showed that serum miR-139-5p level could serve as an independent prognostic marker for OS.

CONCLUSIONS

Taken together, data from this study demonstrates that serum miR-139-5p could be used as a tumor biomarker for OS diagnosis and prognosis.

摘要

背景

微小 RNA（miR-139-5p）在多种肿瘤类型的发生和发展中起着重要作用，包括骨肉瘤（OS）。

目的

本研究旨在检测 OS 患者血清 miR-139-5p 的表达，并分析其与临床变量的关系。

方法

采集 98 例 OS 患者和 50 例健康个体的血样，通过定量逆转录-聚合酶链反应（qRT-PCR）检测血清 miR-139-5p 水平。

结果

我们发现 OS 患者血清 miR-139-5p 的表达明显低于健康个体，且远处转移或临床分期较高的患者 miR-139-5p 水平显著降低。受试者工作特征（ROC）分析表明，血清 miR-139-5p 能够很好地区分 OS 患者与健康对照者，具有较高的灵敏度和特异性。此外，低血清 miR-139-5p 表达与远处转移、肿瘤分期和总生存期较短密切相关。单因素和多因素分析均表明，血清 miR-139-5p 水平可作为 OS 的独立预后标志物。