含胸腺嘧啶光二聚体的双链体d(GCGTTGCG).d(CGCAACGC)可交换质子的1H核磁共振研究
1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.
作者信息
Kemmink J, Boelens R, Koning T, van der Marel G A, van Boom J H, Kaptein R
出版信息
Nucleic Acids Res. 1987 Jun 11;15(11):4645-53. doi: 10.1093/nar/15.11.4645.
Abstract
A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13 degrees C on thymine dimer formation.
摘要
对双链八聚体d(GCGTTGCG).d(CGCAACGC) 及其两个中心胸腺嘧啶残基以顺式-顺式胸腺嘧啶二聚体形式存在的相同八聚体的亚氨基质子核磁共振谱进行了比较。除末端碱基对外，所有亚氨基质子均在核磁共振谱中被检测到并进行了归属。光谱表明，在胸腺嘧啶二聚体双链体中，与普遍看法相反，所有碱基对均以氢键形式存在，尽管两个中心AT碱基对的氢键被大幅削弱。形成胸腺嘧啶二聚体时，解链温度降低约13摄氏度。
相似文献
1
1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer.含胸腺嘧啶光二聚体的双链体d(GCGTTGCG).d(CGCAACGC)可交换质子的1H核磁共振研究
Nucleic Acids Res. 1987 Jun 11;15(11):4645-53. doi: 10.1093/nar/15.11.4645.
2
Conformational changes in the oligonucleotide duplex d(GCGTTGCG) x d(CGCAACGC) induced by formation of a cis-syn thymine dimer. A two-dimensional NMR study.顺式-反式胸腺嘧啶二聚体形成诱导的寡核苷酸双链体d(GCGTTGCG)×d(CGCAACGC)的构象变化。二维核磁共振研究。
Eur J Biochem. 1987 Jan 2;162(1):37-43. doi: 10.1111/j.1432-1033.1987.tb10538.x.
3
Photoreactivation of the thymine dimer containing DNA octamer d(GCGTTGCG).d(CGCAACGC) by the photoreactivating enzyme from Anacystis nidulans.来自集胞藻的光复活酶对含有胸腺嘧啶二聚体的DNA八聚体d(GCGTTGCG).d(CGCAACGC)的光复活作用
J Photochem Photobiol B. 1988 Mar;1(3):323-8. doi: 10.1016/1011-1344(88)85020-6.
4
1H NMR assignment and melting temperature study of cis-syn and trans-syn thymine dimer containing duplexes of d(CGTATTATGC).d(GCATAATACG).含顺式-顺式和反式-顺式胸腺嘧啶二聚体的d(CGTATTATGC).d(GCATAATACG)双链体的1H NMR归属及解链温度研究
Biochemistry. 1990 Sep 18;29(37):8858-66. doi: 10.1021/bi00489a049.
5
Base pair opening in a deoxynucleotide duplex containing a cis-syn thymine cyclobutane dimer lesion.含有顺式-同型胸腺嘧啶环丁烷二聚体损伤的脱氧核苷酸双链体中的碱基对打开。
Biochemistry. 2013 Dec 23;52(51):9275-85. doi: 10.1021/bi401312r. Epub 2013 Dec 11.
6
Nuclear magnetic resonance study of the interaction of T4 endonuclease V with DNA.T4核酸内切酶V与DNA相互作用的核磁共振研究
Biochemistry. 1994 Jan 11;33(1):57-64. doi: 10.1021/bi00167a008.
7
Synthesis and characterization of a [3-15N]-labeled cis-syn thymine dimer-containing DNA duplex.含[3-15N]标记的顺式-胸腺嘧啶二聚体的DNA双链体的合成与表征
J Org Chem. 2006 Feb 17;71(4):1640-6. doi: 10.1021/jo0524167.
8
Synthesis and proton-NMR studies of oligonucleotides containing an apurinic (AP) site.含脱嘌呤（AP）位点的寡核苷酸的合成及质子核磁共振研究
J Biomol Struct Dyn. 1987 Oct;5(2):219-47. doi: 10.1080/07391102.1987.10506391.
9
Proton nuclear magnetic resonance investigation of the conformation and dynamics in the synthetic deoxyribonucleic acid decamers d(ATATCGATAT) and d(ATATGCATAT).合成脱氧核糖核酸十聚体d(ATATCGATAT)和d(ATATGCATAT)的构象与动力学的质子核磁共振研究
Biochemistry. 1983 Dec 6;22(25):5930-42. doi: 10.1021/bi00294a037.
10
Proton nuclear magnetic resonance investigations and ring current calculations of guanine N-1 and thymine N-3 hydrogen-bonded protons in double-helical deoxyribonucleotides in aqueous solution.水溶液中双螺旋脱氧核糖核苷酸中鸟嘌呤N-1和胸腺嘧啶N-3氢键质子的质子核磁共振研究及环电流计算
Proc Natl Acad Sci U S A. 1974 May;71(5):1945-8. doi: 10.1073/pnas.71.5.1945.
引用本文的文献
1
The interplay of supercoiling and thymine dimers in DNA.DNA 中超螺旋和胸腺嘧啶二聚体的相互作用。
Nucleic Acids Res. 2022 Mar 21;50(5):2480-2492. doi: 10.1093/nar/gkac082.
2
A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζ.DNA 聚合酶 λ在与 DNA 聚合酶 ζ 共同进行跨损伤合成中的新作用。
Life Sci Alliance. 2021 Jan 29;4(4). doi: 10.26508/lsa.202000900. Print 2021 Apr.
3
Genetic Control of Replication through N1-methyladenine in Human Cells.人类细胞中通过N1-甲基腺嘌呤对复制的遗传控制。
J Biol Chem. 2015 Dec 11;290(50):29794-800. doi: 10.1074/jbc.M115.693010. Epub 2015 Oct 21.
4
Base pair opening in a deoxynucleotide duplex containing a cis-syn thymine cyclobutane dimer lesion.含有顺式-同型胸腺嘧啶环丁烷二聚体损伤的脱氧核苷酸双链体中的碱基对打开。
Biochemistry. 2013 Dec 23;52(51):9275-85. doi: 10.1021/bi401312r. Epub 2013 Dec 11.
5
Thymine photodimer formation in DNA hairpins. Unusual conformations favor (6 - 4) vs. (2 + 2) adducts.胸腺嘧啶二聚体在 DNA 发夹中的形成。不寻常的构象有利于（6-4）加合物而不是（2+2）加合物。
Photochem Photobiol Sci. 2014 Feb;13(2):266-71. doi: 10.1039/c3pp50283j.
6
Error-free replicative bypass of (6-4) photoproducts by DNA polymerase zeta in mouse and human cells.DNA 聚合酶 ζ 在小鼠和人细胞中无差错复制性跨越（6-4）光产物。
Genes Dev. 2010 Jan 15;24(2):123-8. doi: 10.1101/gad.1872810.
7
Investigating the biochemical impact of DNA damage with structure-based probes: abasic sites, photodimers, alkylation adducts, and oxidative lesions.利用基于结构的探针研究DNA损伤的生化影响：无碱基位点、光二聚体、烷基化加合物和氧化损伤。
Biochemistry. 2009 Oct 13;48(40):9347-59. doi: 10.1021/bi901059k.
8
Nonlinearity in DNA and its relation to specific functions.
J Biol Phys. 2009 Feb;35(1):31-41. doi: 10.1007/s10867-009-9125-4. Epub 2009 Feb 25.
9
Thymine dimer-induced structural changes to the DNA duplex examined with reactive probes (†).用反应性探针检测胸腺嘧啶二聚体诱导的DNA双链结构变化(†)。
Biochemistry. 2008 Dec 9;47(49):13026-35. doi: 10.1021/bi801417u.
10
Synthesis and characterization of a [3-15N]-labeled cis-syn thymine dimer-containing DNA duplex.含[3-15N]标记的顺式-胸腺嘧啶二聚体的DNA双链体的合成与表征
J Org Chem. 2006 Feb 17;71(4):1640-6. doi: 10.1021/jo0524167.
本文引用的文献
1
Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from baker's yeast.用来自面包酵母的光复活酶处理后，紫外线照射的DNA中胸腺嘧啶光二聚体的消失
Biochem Biophys Res Commun. 1962 Apr 20;7:237-40. doi: 10.1016/0006-291x(62)90181-x.
2
The structure of d(CGCGAAT[]TCGCG) . d(CGCGAATTCGCG); the incorporation of a thymine photodimer into a B-DNA helix.d（CGCGAAT[]TCGCG）. d（CGCGAATTCGCG）的结构；胸腺嘧啶光二聚体掺入B-DNA螺旋结构中。
Nucleic Acids Res. 1984 Jun 11;12(11):4789-807. doi: 10.1093/nar/12.11.4789.
3
Effect of a single thymine photodimer on the oligodeoxythymidylate-polydeoxyadenylate interaction.单个胸腺嘧啶光二聚体对寡聚脱氧胸苷酸-聚脱氧腺苷酸相互作用的影响。
J Am Chem Soc. 1971 Sep;93(19):4940-2. doi: 10.1021/ja00748a065.
4
Hairpin formation in synthetic oligonucleotides.合成寡核苷酸中的发夹结构形成。
Biochimie. 1985 Jul-Aug;67(7-8):685-95. doi: 10.1016/s0300-9084(85)80156-5.
5
Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T).碱基-碱基错配。dCA3XA3G + dCT3YT3G（X、Y = A、C、G、T）双螺旋形成的热力学
Nucleic Acids Res. 1985 Jul 11;13(13):4811-24. doi: 10.1093/nar/13.13.4811.
6
Molecular models for DNA damaged by photoreaction.光反应损伤DNA的分子模型。
Science. 1985 Mar 15;227(4692):1304-8. doi: 10.1126/science.3975615.
Zotero 插件