FLIRT：用于亚细胞水平蛋白质功能控制的快速局部红外热基因技术。
FLIRT: fast local infrared thermogenetics for subcellular control of protein function.
机构信息
Department of Genetics and Development, Columbia University Medical Center, New York, NY, USA.
Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.
出版信息
Nat Methods. 2018 Nov;15(11):921-923. doi: 10.1038/s41592-018-0168-y. Epub 2018 Oct 30.
FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in Caenorhabditis elegans with cell-specific and even subcellular precision.
FLIRT（快速局部红外热遗传学）是一种基于显微镜的技术，可在体内同时监测时局部且可逆地操纵蛋白质功能。FLIRT 可局部使快速作用的温度敏感突变蛋白失活。我们证明，FLIRT 可以控制细胞分裂、Delta-Notch 细胞命运信号传导和秀丽隐杆线虫生殖系结构所必需的温度敏感蛋白，具有细胞特异性甚至亚细胞精度。
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