Wang Chao, Sargsyan Davit, Zhang Chengyue, Wu Renyi, Yang Yuqing, Kong Ah-Ng
Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
Center for Phytochemical Epigenome Studies, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ, U.S.A.
Anticancer Res. 2018 Nov;38(11):6069-6083. doi: 10.21873/anticanres.12957.
BACKGROUND/AIM: Transcriptomic analysis was performed to evaluate the differential gene expression profiles of Setd7 knockdown (KD) and the effects of phenethyl isothiocyanate (PEITC) in human prostate cancer (PCa) LNCaP cells.
RNA isolated from wild-type and Setd7-KD LNCaP cells in the presence or absence of PEITC was subjected to microarray analysis followed by Ingenuity® Pathway Analysis (IPA).
Setd7 KD impacted a larger set of genes and caused a higher fold change compared to PEITC treatment. Several signaling pathways were altered particularly inflammation-related TNFR signaling and PTEN/PI3K/AKT signaling by Setd7 KD and PEITC. Interestingly, PEITC and Setd7 KD at a small subset of genes that could be potential molecular targets.
This study offers new insights into the mechanisms of action of the epigenetic modifier Setd7 and the effects of PEITC treatment in PCa cells and enhances our understanding of the potential cancer preventive/treatment effects of isothiocyanate compounds such as PEITC in PCa.
背景/目的：进行转录组分析以评估Setd7基因敲低（KD）的差异基因表达谱以及异硫氰酸苯乙酯（PEITC）对人前列腺癌（PCa）LNCaP细胞的影响。
从存在或不存在PEITC的野生型和Setd7-KD LNCaP细胞中分离的RNA进行微阵列分析，随后进行Ingenuity®通路分析（IPA）。
与PEITC处理相比，Setd7基因敲低影响的基因集更大且导致的倍数变化更高。Setd7基因敲低和PEITC改变了几个信号通路，特别是与炎症相关的TNFR信号通路和PTEN/PI3K/AKT信号通路。有趣的是，PEITC和Setd7基因敲低作用于一小部分可能成为潜在分子靶点的基因。
本研究为表观遗传修饰因子Setd7的作用机制以及PEITC处理对PCa细胞的影响提供了新见解，并增强了我们对异硫氰酸酯化合物如PEITC在PCa中潜在的癌症预防/治疗作用的理解。
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