指尖上的癌症检测：基于智能手机的DNA检测

Cancer Detection at your Fingertips: Smartphone-Enabled DNA Testing.

作者信息

Turner Robert, Madsen James, Herrera Pedro David Simon, Wallace John, Madrigal Jonathan, Hinestrosa Juan Pablo, Dobrovolskaia Irina, Krishnan Rajaram

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul;2018:5418-5421. doi: 10.1109/EMBC.2018.8513553.

DOI:10.1109/EMBC.2018.8513553

PMID:30441562

Abstract

High molecular weight cell-free DNA (hmw cfDNA) found in biological fluid, such as blood, is a promising biomarker for cancer detection. Due to the abundance of background apoptotic cell-free DNA in blood, quantifying the native concentration of hmw cfDNA using existing methods is technically challenging, time consuming, and expensive. We have developed a novel technology which utilizes Alternating Current Electrokinetics (ACE) to isolate hmw cfDNA directly from blood. Furthermore, we integrated this technology into a handheld device which utilizes a smartphone for power, instruction transmission, optical detection, image processing, and data transmission. The detection of hmw cfDNA in blood plasma demonstrated the performance of the device. We are continuing development of this device as a future point of care in vitro diagnostic.

摘要

在血液等生物流体中发现的高分子量游离DNA（hmw cfDNA）是一种很有前景的癌症检测生物标志物。由于血液中存在大量背景凋亡游离DNA，使用现有方法定量hmw cfDNA的天然浓度在技术上具有挑战性，耗时且昂贵。我们开发了一种利用交流电动力学（ACE）直接从血液中分离hmw cfDNA的新技术。此外，我们将该技术集成到了一个手持设备中，该设备利用智能手机进行供电、指令传输、光学检测、图像处理和数据传输。血浆中hmw cfDNA的检测证明了该设备的性能。我们正在继续开发该设备，将其作为未来的即时医疗体外诊断工具。