印度南部选定猪群中布鲁氏菌病的血清学和分子分析。
Serological and molecular analysis for brucellosis in selected swine herds from Southern India.
机构信息
Indian Council of Agricultural Research, National Institute of Veterinary Epidemiology and Disease Informatics, Ramagondanahalli, Bengaluru, 560064, India.
Indian Council of Agricultural Research, National Institute of Veterinary Epidemiology and Disease Informatics, Ramagondanahalli, Bengaluru, 560064, India.
出版信息
J Infect Public Health. 2019 Mar-Apr;12(2):247-251. doi: 10.1016/j.jiph.2018.10.013. Epub 2018 Nov 15.
BACKGROUND
Swine brucellosis is a zoonotic disease caused by Brucella suis. The study describes the occurrence of brucellosis in two organized piggeries in Southern India.
METHODS
A total of 585 serum samples comprising 575 from pigs and 10 from animal handlers were collected and tested by serological tests and PCR. Tissue samples were collected for isolation of the pathogen.
RESULTS
Out of 575 serum samples screened, 236 (41.04%) were positive for brucellosis by both Rose Bengal plate test (RBPT) and indirect ELISA (iELISA) and 47 (8.17) samples showed Brucella DNA amplification by genus specific PCR. The sows those aborted and 19 boars with orchitis were seropositive for brucellosis indicating association of clinical symptoms with brucellosis seropositivity. Two of 10 pig handlers were positive by RBPT and showed significant serum agglutination test (SAT) titres of >1:160 and 1:320. B. suis bvI was isolated and identified by biochemical tests and confirmed by amplification Brucella genus and Bruce ladder PCRs from vaginal and testicular samples.
CONCLUSIONS
The introduction of untested breeding boars in the farms might have resulted in the disease transmission and spread. The present study highlighted the diagnosis of B. suis bvI as a cause of abortions in the pigs and occupational exposure to farm personnel.
背景
猪布鲁氏菌病是一种由猪布鲁氏菌引起的人畜共患病。本研究描述了印度南部两个有组织的养猪场发生的布鲁氏菌病。
方法
共采集了 585 份血清样本，其中 575 份来自猪，10 份来自动物饲养员，通过血清学试验和 PCR 进行检测。采集组织样本用于分离病原体。
结果
在筛查的 575 份血清样本中，236 份（41.04%）经虎红平板试验（RBPT）和间接酶联免疫吸附试验（iELISA）均呈布鲁氏菌病阳性，47 份（8.17%）样本经属特异性 PCR 显示布鲁氏菌 DNA 扩增。流产的母猪和 19 头睾丸炎公猪血清布鲁氏菌病阳性，表明临床症状与布鲁氏菌病血清阳性有关。10 名饲养员中有 2 人 RBPT 阳性，血清凝集试验（SAT）滴度>1:160 和 1:320 显著。通过生化试验从阴道和睾丸样本中分离和鉴定出 B. suis bvI，并通过扩增布鲁氏菌属和布鲁斯梯式 PCR 进行确认。
结论
未经过检测的繁殖公猪引入农场可能导致了疾病的传播和扩散。本研究强调了 B. suis bvI 作为导致猪流产的原因以及农场工作人员职业暴露的诊断。
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