Fatsa Faculty of Marine Science, Department of Marine Science and Technology Engineering, Ordu University, 52200 Ordu, Turkey.
Int J Biol Macromol. 2019 Feb 15;123:878-888. doi: 10.1016/j.ijbiomac.2018.11.143. Epub 2018 Nov 15.
In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride. HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined. The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied. It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased. Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher. The mechanical properties of the films were affected by the amount of HA and the A:G ratio. Incorporation of HA increased the thermal stability of films. The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased. It was found that films containing TH showed slightly higher antimicrobial activity against Staphylococcus aureus than Escherichia coli.
在这项研究中，通过溶液浇铸法制备了含有羟磷灰石 (HA) 的藻酸盐-明胶薄膜，将藻酸盐 (A) 和明胶 (G) 溶液混合，然后用氯化钙交联。将 HA（1、2、5、10、20%w/w）分别添加到以不同比例（A:G=40:60、50:50 和 60:40）制备的薄膜溶液中，并研究了所得薄膜的溶胀和降解行为、力学性能、抗菌性能和热性能以及形貌。研究了从制备的薄膜中释放盐酸四环素（TH）的情况。结果表明，随着藻酸盐和 HA 用量的增加，薄膜的溶胀率和失重率降低。扫描电子显微镜分析表明，随着薄膜中 HA 含量的增加，薄膜表面变得更加粗糙。薄膜的力学性能受 HA 用量和 A:G 比的影响。HA 的加入提高了薄膜的热稳定性。在 15 分钟内从薄膜中释放的 TH 量随着藻酸盐和羟磷灰石用量的增加而减少。结果发现，含有 TH 的薄膜对金黄色葡萄球菌的抗菌活性略高于大肠杆菌。
