Department of Energy, Joint Genome Institute, CA 94598, USA.
Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Future Microbiol. 2018 Dec;13:1709-1717. doi: 10.2217/fmb-2018-0267. Epub 2018 Nov 27.
To describe the genomic characteristics of seawater-borne hemolytic Shewanella algae and its resistance genes.
MATERIALS & METHODS: Whole genome sequence of S. algae SYT3 was determined using llumina MiSeq platform. Multiple-database-based analysis was performed to identify the genetic background of its hemolytic activity and the antibiotic resistance genes.
S. algae SYT3 possesses a homolog of the hly operon involved in the synthesis of hemolysin. We also identified candidate genes associated with resistance to β-lactam antibiotics (bla ) and fluoroquinolone (qnrA3).
The study provides an insight into the hemolytic activity of S. algae. Our findings also suggested S. algae as a potential reservoir of antimicrobial resistance determinants.
描述海水源性溶血性希瓦氏菌的基因组特征及其耐药基因。
采用 Illumina MiSeq 平台对 SYT3 进行全基因组测序。采用多数据库分析方法，确定其溶血活性和抗生素耐药基因的遗传背景。
SYT3 含有与溶血素合成有关的 hly 操纵子的同源物。我们还鉴定了与β-内酰胺类抗生素（bla）和氟喹诺酮类（qnrA3）耐药相关的候选基因。
该研究深入了解了希瓦氏菌的溶血活性。我们的研究结果还表明，希瓦氏菌可能是一种潜在的抗生素耐药决定因素库。
