从海水中分离的鞘氨醇单胞菌 SYT3 的全基因组特征揭示了溶血的机制。
Whole-genome characterization of Shewanella algae strain SYT3 isolated from seawater reveals insight into hemolysis.
机构信息
Department of Energy, Joint Genome Institute, CA 94598, USA.
Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
出版信息
Future Microbiol. 2018 Dec;13:1709-1717. doi: 10.2217/fmb-2018-0267. Epub 2018 Nov 27.
AIM
To describe the genomic characteristics of seawater-borne hemolytic Shewanella algae and its resistance genes.
MATERIALS & METHODS: Whole genome sequence of S. algae SYT3 was determined using llumina MiSeq platform. Multiple-database-based analysis was performed to identify the genetic background of its hemolytic activity and the antibiotic resistance genes.
RESULTS
S. algae SYT3 possesses a homolog of the hly operon involved in the synthesis of hemolysin. We also identified candidate genes associated with resistance to β-lactam antibiotics (bla ) and fluoroquinolone (qnrA3).
CONCLUSION
The study provides an insight into the hemolytic activity of S. algae. Our findings also suggested S. algae as a potential reservoir of antimicrobial resistance determinants.
目的
描述海水源性溶血性希瓦氏菌的基因组特征及其耐药基因。
材料与方法
采用 Illumina MiSeq 平台对 SYT3 进行全基因组测序。采用多数据库分析方法，确定其溶血活性和抗生素耐药基因的遗传背景。
结果
SYT3 含有与溶血素合成有关的 hly 操纵子的同源物。我们还鉴定了与β-内酰胺类抗生素（bla）和氟喹诺酮类（qnrA3）耐药相关的候选基因。
结论
该研究深入了解了希瓦氏菌的溶血活性。我们的研究结果还表明，希瓦氏菌可能是一种潜在的抗生素耐药决定因素库。
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