Department of Diagnosis and Surgery, School of Dentistry at Araraquara - Univ Estadual Paulista (UNESP), Rua Humaita, 1680 - Centro, Araraquara, SP, 14801-903, Brazil.
Department of Clinical and Social Dentistry- Health Science Center, Federal University of Paraíba, João Pessoa, Brazil.
Clin Oral Investig. 2019 Aug;23(8):3297-3306. doi: 10.1007/s00784-018-2755-9. Epub 2018 Nov 29.
Studies have documented the anti-inflammatory effects of spices, which may be related to treatment of chronic diseases. The purpose of this study was to evaluate the influence of curcumin and piperine and their association on experimental periodontal repair in rats.
Periodontitis was induced via the installation of a ligature around the first molar. After 15 days, the ligatures were removed, and the rats were separated into groups (12 animals per group): (i) curcumin, (ii) piperine, (iii) curcumin+piperine, (iv) corn oil vehicle, and (v) control group (animals had ligature-induced periodontitis but were not treated). The compounds were administered daily, for 15 days by oral gavage. Animals were euthanized at 5 and 15 days, and hemimaxillae and gingival tissues were harvested. Bone repair was assessed by μCT (microcomputer tomography). Histological sections were stained with hematoxylin/eosin (H/E) for the assessment of cellular infiltrate or picrosirius red for quantification of collagen content, and subjected to immunohistochemistry for detecting NF-ĸB. Gingival tissues were used to evaluate levels of TGF-β and IL-10 (ELISA).
Curcumin and piperine increased the TGF-β level, significantly improved the collagen repair, and decreased the cellularity and activation of NF-ĸB in the periodontal tissues, but only curcumin caused a significant increase in early bone repair.
Curcumin and piperine promoted a substantive effect on tissue repair; however, there was not synergistic effect of compounds administered in combination.
Curcumin and piperine stimulates the tissue repair and may be potential candidates for the treatment of periodontal disease.
有研究记载了香料的抗炎作用，这可能与慢性病的治疗有关。本研究旨在评估姜黄素和胡椒碱及其联合应用对大鼠实验性牙周修复的影响。
通过在第一磨牙周围安装结扎线来诱导牙周炎。15 天后，去除结扎线，将大鼠分为五组（每组 12 只动物）：（i）姜黄素，（ii）胡椒碱，（iii）姜黄素+胡椒碱，（iv）玉米油载体，和（v）对照组（动物有结扎诱导的牙周炎但未治疗）。这些化合物通过口服灌胃每天给药，共 15 天。动物在第 5 天和第 15 天处死，采集上颌骨和牙龈组织。通过微计算机断层扫描（microcomputer tomography，μCT）评估骨修复。用苏木精/伊红（hematoxylin/eosin，H/E）染色进行细胞浸润评估，或用苦味酸天狼猩红染色进行胶原含量定量，并进行 NF-κB 的免疫组织化学染色。用牙龈组织进行转化生长因子-β（transforming growth factor-β，TGF-β）和白细胞介素-10（interleukin-10，IL-10）水平的酶联免疫吸附测定（ELISA）。
姜黄素和胡椒碱增加了 TGF-β水平，显著改善了胶原修复，并减少了牙周组织中的细胞浸润和 NF-κB 的激活，但只有姜黄素显著增加了早期骨修复。
姜黄素和胡椒碱对组织修复有显著作用，但联合应用化合物没有协同作用。
姜黄素和胡椒碱刺激组织修复，可能是牙周病治疗的潜在候选药物。
