Wang Siran, Li Junfeng, Dong Zhihao, Chen Lei, Shao Tao
Institute of Ensiling and Processing of Grass, College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, China.
Anim Sci J. 2019 Feb;90(2):178-188. doi: 10.1111/asj.13134. Epub 2018 Dec 6.
This study was aimed to examine the characteristics of three isolated lactic acid bacteria (LAB) strains (HG24, M1, and TG1), and to evaluate their effects on fermentation characteristics, nutritive value, and in vitro digestibility in a variety of substrates. The experiments were conducted with Italian ryegrass (Lolium multiflorum Lam.), tall fescue (Festuca arundinacea Schred.), and oat (Avena sativa L.) in laboratory silo (1 L) for ensiling 30 days. All the treated silages had significantly (p < 0.05) higher in vitro dry matter digestibility (IVDMD), and lower undesirable microorganism populations compared with controls. In Italian ryegrass and oat silages, all the inoculants improved the fermentation quality, evidenced by distinctly (p < 0.05) higher LA contents and ratios of LA to acetic acid, and lower pH, butyric acid and ammonia nitrogen contents. Strains HG24 and commercial inoculant G (Lactobacillus plantarum MTD-1) performed better than strains M1 and TG1, indicated by obviously (p < 0.05) higher LA contents, LAB numbers, and IVDMD. In tall fescue silage, all the isolated LAB strains (HG24, M1 and TG1) markedly (p < 0.05) increased IVDMD compared with strain G. Strain HG24 is recommended as a good LAB inoculant for ensiling in various forages.
本研究旨在检测三株分离的乳酸菌(LAB)菌株(HG24、M1和TG1)的特性,并评估它们对多种底物发酵特性、营养价值和体外消化率的影响。实验采用意大利黑麦草(多花黑麦草)、高羊茅和燕麦,在1升实验室青贮窖中进行30天青贮。与对照相比,所有处理的青贮料体外干物质消化率(IVDMD)显著更高(p<0.05),且不良微生物数量更低。在意大利黑麦草和燕麦青贮料中,所有接种剂均改善了发酵品质,表现为乳酸(LA)含量及LA与乙酸的比例显著更高(p<0.05),pH值、丁酸和氨态氮含量更低。菌株HG24和商业接种剂G(植物乳杆菌MTD-1)的表现优于菌株M1和TG1,表现为LA含量、乳酸菌数量和IVDMD显著更高(p<0.05)。在高羊茅青贮料中,与接种剂G相比,所有分离的LAB菌株(HG24、M1和TG1)均显著提高了IVDMD(p<0.05)。菌株HG24被推荐为用于各种草料青贮的优良LAB接种剂。