利用原位生长的功能化 CuSe/还原氧化石墨烯杂化材料作为高效纳米酶进行癌细胞的可视化检测。
Visual detection of cancer cells by using in situ grown functional CuSe/reduced graphene oxide hybrids acting as an efficient nanozyme.
机构信息
Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, P. R. China.
College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.
出版信息
Analyst. 2019 Jan 21;144(2):716-721. doi: 10.1039/c8an01849a. Epub 2018 Dec 6.
A simple protocol for in situ growth of CuSe nanoparticles on graphene oxide hybrids (CuSe/rGO) acting as an efficient nanozyme is developed and thus a sensitive visual detection method of cancer cells is proposed. The CuSe/rGO heterogeneous nanomaterials have been proven to exhibit high peroxidase-like activity to catalyze the reaction of the peroxidase substrate in the presence of hydrogen peroxide. Herein, we used CuSe/rGO as a signal transducer to develop a colorimetric assay for the direct detection of cancer cells and a total of 63 cancer cells (MCF-7) can be distinguished by naked-eye observation. The results showed that the CuSe/rGO hybrids could be the promising nanozyme mimetics for potential applications in bioanalytical fields.
我们开发了一种简单的方案，用于在氧化石墨烯杂化物（CuSe/rGO）上原位生长 CuSe 纳米粒子，其可作为有效的纳米酶，从而提出了一种用于癌细胞灵敏可视化检测的新方法。已证实，CuSe/rGO 异质纳米材料具有类过氧化物酶的高活性，可以在存在过氧化氢的情况下催化过氧化物酶底物的反应。在此，我们使用 CuSe/rGO 作为信号转导物，开发了用于直接检测癌细胞的比色分析方法，通过肉眼观察可以区分总共 63 个癌细胞（MCF-7）。结果表明，CuSe/rGO 杂化物有望成为潜在应用于生物分析领域的有前途的纳米酶模拟物。
Zotero 插件