夹竹桃科罗布麻花中两种新型多糖的抗凝活性
Anticoagulant activity of two novel polysaccharides from flowers of Apocynum venetum L.
机构信息
National Center for Research and Development of Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China.
Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Kaifeng 475004, China; Medicinal and Aromatic Plants Research Department, Horticulture Research Institute, Agricultural Research Center, Egypt.
出版信息
Int J Biol Macromol. 2019 Mar 1;124:1230-1237. doi: 10.1016/j.ijbiomac.2018.12.015. Epub 2018 Dec 3.
Two novel polysaccharides were obtained from flowers of Apocynum venetum L., and named as Vp2a-II and Vp3. Their average molecular weights were 7 kDa and 9 kDa, respectively. Methods of monosaccharide analysis, acid hydrolysis and methylation analysis, Fourier transform-infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy were used to identify the structure of Vp2a-II and Vp3. Vp2a-II was composed of →6)-β-d-Glcp-(1 → 6)-α-d-Galp-(1→ residues. Vp3 was composed of α-d-GlcpA-(3 → α-d-GalpA residues. The anticoagulant activity was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays in vitro. Results indicated that Vp3 exhibited the anticoagulant activity.
从罗布麻的花中得到了两种新型多糖，分别命名为 Vp2a-II 和 Vp3。它们的平均分子量分别为 7 kDa 和 9 kDa。采用单糖分析、酸水解和甲基化分析、傅里叶变换红外光谱（FT-IR）、气相色谱-质谱联用（GC-MS）和核磁共振（NMR）光谱等方法鉴定了 Vp2a-II 和 Vp3 的结构。Vp2a-II 由→6）-β-d-Glcp-（1→6）-α-d-Galp-（1→残基组成。Vp3 由α-d-GlcpA-（3→α-d-GalpA 残基组成。通过体外激活部分凝血活酶时间（APTT）、凝血酶时间（TT）、凝血酶原时间（PT）和纤维蛋白原（FIB）测定评估抗凝活性。结果表明 Vp3 具有抗凝活性。
Zotero 插件