RNA 结合蛋白 Zfp36l1 和 Zfp36l2 在成肌分化中冗余发挥作用。

The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.

作者信息

Hema Bye-A-Jee, Dhamayanthi Pugazhendhi, Samuel Woodhouse, Patrick Brien, Rachel Watson, Martin Turner, Jennifer Pell

机构信息

Laboratory of Cell Signalling, The Babraham Institute, Cambridge, UK.

Present address: European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, UK.

出版信息

Skelet Muscle. 2018 Dec 7;8(1):37. doi: 10.1186/s13395-018-0183-9.

DOI:10.1186/s13395-018-0183-9

PMID:30526691

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6286576/

Abstract

BACKGROUND Members of the ZFP36 family of RNA-binding proteins regulate gene expression post-transcriptionally by binding to AU-rich elements in the 3'UTR of mRNA and stimulating mRNA degradation. The proteins within this family target different transcripts in different tissues. In particular, ZFP36 targets myogenic transcripts and may have a role in adult muscle stem cell quiescence. Our study examined the requirement of ZFP36L1 and ZFP36L2 in adult muscle cell fate regulation. METHODS We generated single and double conditional knockout mice in which Zfp36l1 and/or Zfp36l2 were deleted in Pax7-expressing cells. Immunostained muscle sections were used to analyse resting skeletal muscle, and a cardiotoxin-induced injury model was used to determine the regenerative capacity of muscle. RESULTS We show that ZFP36L1 and ZFP36L2 proteins are expressed in satellite cells. Mice lacking the two proteins in Pax7-expressing cells have reduced body weight and have reduced skeletal muscle mass. Furthermore, the number of satellite cells is reduced in adult skeletal muscle and the capacity of this muscle to regenerate following muscle injury is diminished. CONCLUSION ZFP36L1 and ZFP36L2 act redundantly in myogenesis. These findings add further intricacy to the regulation of the cell fate of Pax7-expressing cells in skeletal muscle by RNA-binding proteins.

摘要

背景 ZFP36 家族的 RNA 结合蛋白成员通过结合 mRNA 3'UTR 中的 AU 丰富元件并刺激 mRNA 降解，在后转录水平上调节基因表达。该家族内的蛋白质在不同组织中靶向不同的转录本。特别是，ZFP36 靶向肌源性转录本，可能在成体肌肉干细胞静止中发挥作用。我们的研究检查了 ZFP36L1 和 ZFP36L2 在成体肌肉细胞命运调节中的需求。 方法 我们生成了单和双条件敲除小鼠，其中 Zfp36l1 和/或 Zfp36l2 在 Pax7 表达细胞中被删除。使用免疫染色的肌肉切片分析静止的骨骼肌，并使用心脏毒素诱导的损伤模型来确定肌肉的再生能力。 结果 我们表明 ZFP36L1 和 ZFP36L2 蛋白在卫星细胞中表达。在 Pax7 表达细胞中缺乏这两种蛋白质的小鼠体重减轻，骨骼肌质量减少。此外，成年骨骼肌中的卫星细胞数量减少，并且这种肌肉在肌肉损伤后再生的能力降低。 结论 ZFP36L1 和 ZFP36L2 在成肌作用中具有冗余作用。这些发现为 RNA 结合蛋白对骨骼肌中 Pax7 表达细胞的细胞命运的调节增加了更多的复杂性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9273/6286576/ca6bbb7bad9f/13395_2018_183_Fig1_HTML.jpg

正在获取文献详情

最多等待 10 秒，若超时请稍后重试。

RNA 结合蛋白 Zfp36l1 和 Zfp36l2 在成肌分化中冗余发挥作用。

The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.

作者信息

Hema Bye-A-Jee, Dhamayanthi Pugazhendhi, Samuel Woodhouse, Patrick Brien, Rachel Watson, Martin Turner, Jennifer Pell

机构信息

Laboratory of Cell Signalling, The Babraham Institute, Cambridge, UK.

Present address: European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, UK.

出版信息

Skelet Muscle. 2018 Dec 7;8(1):37. doi: 10.1186/s13395-018-0183-9.

DOI:10.1186/s13395-018-0183-9

PMID:30526691

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6286576/

Abstract

BACKGROUND Members of the ZFP36 family of RNA-binding proteins regulate gene expression post-transcriptionally by binding to AU-rich elements in the 3'UTR of mRNA and stimulating mRNA degradation. The proteins within this family target different transcripts in different tissues. In particular, ZFP36 targets myogenic transcripts and may have a role in adult muscle stem cell quiescence. Our study examined the requirement of ZFP36L1 and ZFP36L2 in adult muscle cell fate regulation. METHODS We generated single and double conditional knockout mice in which Zfp36l1 and/or Zfp36l2 were deleted in Pax7-expressing cells. Immunostained muscle sections were used to analyse resting skeletal muscle, and a cardiotoxin-induced injury model was used to determine the regenerative capacity of muscle. RESULTS We show that ZFP36L1 and ZFP36L2 proteins are expressed in satellite cells. Mice lacking the two proteins in Pax7-expressing cells have reduced body weight and have reduced skeletal muscle mass. Furthermore, the number of satellite cells is reduced in adult skeletal muscle and the capacity of this muscle to regenerate following muscle injury is diminished. CONCLUSION ZFP36L1 and ZFP36L2 act redundantly in myogenesis. These findings add further intricacy to the regulation of the cell fate of Pax7-expressing cells in skeletal muscle by RNA-binding proteins.

摘要

背景 ZFP36 家族的 RNA 结合蛋白成员通过结合 mRNA 3'UTR 中的 AU 丰富元件并刺激 mRNA 降解，在后转录水平上调节基因表达。该家族内的蛋白质在不同组织中靶向不同的转录本。特别是，ZFP36 靶向肌源性转录本，可能在成体肌肉干细胞静止中发挥作用。我们的研究检查了 ZFP36L1 和 ZFP36L2 在成体肌肉细胞命运调节中的需求。 方法 我们生成了单和双条件敲除小鼠，其中 Zfp36l1 和/或 Zfp36l2 在 Pax7 表达细胞中被删除。使用免疫染色的肌肉切片分析静止的骨骼肌，并使用心脏毒素诱导的损伤模型来确定肌肉的再生能力。 结果 我们表明 ZFP36L1 和 ZFP36L2 蛋白在卫星细胞中表达。在 Pax7 表达细胞中缺乏这两种蛋白质的小鼠体重减轻，骨骼肌质量减少。此外，成年骨骼肌中的卫星细胞数量减少，并且这种肌肉在肌肉损伤后再生的能力降低。 结论 ZFP36L1 和 ZFP36L2 在成肌作用中具有冗余作用。这些发现为 RNA 结合蛋白对骨骼肌中 Pax7 表达细胞的细胞命运的调节增加了更多的复杂性。

Suppr 超能文献

正在获取文献详情

文献检索

文件翻译

深度研究

Suppr 超能文献

正在获取文献详情

正在获取文献详情

文献检索

文件翻译

深度研究

RNA 结合蛋白 Zfp36l1 和 Zfp36l2 在成肌分化中冗余发挥作用。

The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.

作者信息

机构信息

出版信息

BACKGROUND

METHODS

RESULTS

CONCLUSION

背景

方法

结果

结论

正在获取文献详情

相关文献

1The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.PubMed译RNA 结合蛋白 Zfp36l1 和 Zfp36l2 在成肌分化中冗余发挥作用。

2Differential expression and functional analysis of the tristetraprolin family during early differentiation of 3T3-L1 preadipocytes.PubMed译Tristetraprolin 家族在 3T3-L1 前脂肪细胞早期分化过程中的差异表达和功能分析。

3The RNA-Binding Proteins Zfp36l1 and Zfp36l2 Enforce the Thymic β-Selection Checkpoint by Limiting DNA Damage Response Signaling and Cell Cycle Progression.PubMed译RNA结合蛋白Zfp36l1和Zfp36l2通过限制DNA损伤反应信号传导和细胞周期进程来强化胸腺β选择检查点。

4Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development.PubMed译鉴定和描述在出生后发育过程中一种非卫星细胞的肌源性驻留前体细胞。

5The ZFP36 family of RNA binding proteins regulates homeostatic and autoreactive T cell responses.PubMed译ZFP36 家族的 RNA 结合蛋白调节稳态和自身反应性 T 细胞反应。

6Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes.PubMed译胰岛素可增加小鼠3T3-L1脂肪细胞中锌指蛋白36（Tristetraprolin）的表达，并降低血管内皮生长因子（VEGF）基因的表达。

7mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration.PubMed译mTOR对于卫星细胞的正常活动和骨骼肌再生是必需的。

8Bmi1 is expressed in postnatal myogenic satellite cells, controls their maintenance and plays an essential role in repeated muscle regeneration.PubMed译Bmi1 在出生后的肌肉卫星细胞中表达，控制它们的维持，并在反复的肌肉再生中发挥重要作用。

9Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration.PubMed译Pax7 表达的卫星细胞对于成体骨骼肌再生是不可或缺的。

10Post-transcriptional regulation of BCL2 mRNA by the RNA-binding protein ZFP36L1 in malignant B cells.PubMed译RNA结合蛋白ZFP36L1对恶性B细胞中BCL2 mRNA的转录后调控。

相关文献

1ZFP36-family RNA-binding proteins in regulatory T cells reinforce immune homeostasis.PubMed译调节性T细胞中的ZFP36家族RNA结合蛋白增强免疫稳态。

相关文献

1The Future of Cross-Linking and Immunoprecipitation (CLIP).PubMed译交联免疫沉淀（CLIP）的未来。

RNA 结合蛋白 Zfp36l1 和 Zfp36l2 在成肌分化中冗余发挥作用。

The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.

作者信息

机构信息

出版信息

BACKGROUND

METHODS

RESULTS

CONCLUSION

背景

方法

结果

结论

相关文献

1The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.PubMed译RNA 结合蛋白 Zfp36l1 和 Zfp36l2 在成肌分化中冗余发挥作用。

2Differential expression and functional analysis of the tristetraprolin family during early differentiation of 3T3-L1 preadipocytes.PubMed译Tristetraprolin 家族在 3T3-L1 前脂肪细胞早期分化过程中的差异表达和功能分析。

3The RNA-Binding Proteins Zfp36l1 and Zfp36l2 Enforce the Thymic β-Selection Checkpoint by Limiting DNA Damage Response Signaling and Cell Cycle Progression.PubMed译RNA结合蛋白Zfp36l1和Zfp36l2通过限制DNA损伤反应信号传导和细胞周期进程来强化胸腺β选择检查点。

4Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development.PubMed译鉴定和描述在出生后发育过程中一种非卫星细胞的肌源性驻留前体细胞。

5The ZFP36 family of RNA binding proteins regulates homeostatic and autoreactive T cell responses.PubMed译ZFP36 家族的 RNA 结合蛋白调节稳态和自身反应性 T 细胞反应。

6Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes.PubMed译胰岛素可增加小鼠3T3-L1脂肪细胞中锌指蛋白36（Tristetraprolin）的表达，并降低血管内皮生长因子（VEGF）基因的表达。

7mTOR is necessary for proper satellite cell activity and skeletal muscle regeneration.PubMed译mTOR对于卫星细胞的正常活动和骨骼肌再生是必需的。

8Bmi1 is expressed in postnatal myogenic satellite cells, controls their maintenance and plays an essential role in repeated muscle regeneration.PubMed译Bmi1 在出生后的肌肉卫星细胞中表达，控制它们的维持，并在反复的肌肉再生中发挥重要作用。

9Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration.PubMed译Pax7 表达的卫星细胞对于成体骨骼肌再生是不可或缺的。

10Post-transcriptional regulation of BCL2 mRNA by the RNA-binding protein ZFP36L1 in malignant B cells.PubMed译RNA结合蛋白ZFP36L1对恶性B细胞中BCL2 mRNA的转录后调控。

相关文献

1ZFP36-family RNA-binding proteins in regulatory T cells reinforce immune homeostasis.PubMed译调节性T细胞中的ZFP36家族RNA结合蛋白增强免疫稳态。

相关文献

1The Future of Cross-Linking and Immunoprecipitation (CLIP).PubMed译交联免疫沉淀（CLIP）的未来。

2The zinc finger protein ZFP36L2 inhibits flavivirus infection via the 5'-3' XRN1-mediated RNA decay pathway in the replication complexes.PubMed译锌指蛋白ZFP36L2通过复制复合体中5'-3' XRN1介导的RNA衰变途径抑制黄病毒感染。

3LMNA R482L mutation causes impairments in C2C12 myoblasts subpopulations, alterations in metabolic reprogramming during differentiation, and oxidative stress.PubMed译LMNA基因R482L突变导致C2C12成肌细胞亚群受损、分化过程中代谢重编程改变以及氧化应激。

4Synergistic roles of tristetraprolin family members in myeloid cells in the control of inflammation.PubMed译三肽重复蛋白家族成员在髓系细胞中对炎症控制的协同作用。

5Genome-wide epigenetic dynamics during postnatal skeletal muscle growth in Hu sheep.PubMed译绵羊出生后骨骼肌生长过程中的全基因组表观遗传学动态。

7Dynamic chromatin architectures provide insights into the genetics of cattle myogenesis.PubMed译动态染色质结构为牛肌肉生成的遗传学研究提供了见解。

8Deciphering postnatal limb development at single-cell resolution.PubMed译以单细胞分辨率解析出生后肢体发育

9Integrated bioinformatics, network pharmacology, and artificial intelligence to predict the mechanism of celastrol against muscle atrophy caused by colorectal cancer.PubMed译整合生物信息学、网络药理学和人工智能以预测雷公藤红素抗结直肠癌所致肌肉萎缩的机制

10Skeletal Muscle Regeneration in Cardiotoxin-Induced Muscle Injury Models.PubMed译心肌毒素诱导的肌肉损伤模型中的骨骼肌再生。

2Chromatin Modification and Global Transcriptional Silencing in the Oocyte Mediated by the mRNA Decay Activator ZFP36L2.PubMed译mRNA 衰变激活因子 ZFP36L2 介导的卵母细胞染色质修饰和全局转录沉默

3Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells.PubMed译用于分析静止和激活的肌肉干细胞的单根趾长伸肌肌纤维分离

4Inducible satellite cell depletion attenuates skeletal muscle regrowth following a scald-burn injury.PubMed译诱导性卫星细胞耗竭可减轻烫伤烧伤后的骨骼肌再生。

5Maintenance of the marginal-zone B cell compartment specifically requires the RNA-binding protein ZFP36L1.PubMed译边缘区B细胞区室的维持特别需要RNA结合蛋白ZFP36L1。