通过间接免疫荧光法检测抗中性粒细胞胞浆抗体（ANCA）

Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence.

作者信息

van Beers J J B C, Vanderlocht J, Roozendaal C, Damoiseaux J

机构信息

Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, The Netherlands.

Department of Laboratory Medicine, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.

出版信息

Methods Mol Biol. 2019;1901:47-62. doi: 10.1007/978-1-4939-8949-2_4.

DOI:10.1007/978-1-4939-8949-2_4

PMID:30539567

Abstract

The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.

摘要

抗中性粒细胞胞浆抗体（ANCA）的最终检测可首先通过间接免疫荧光法（IIF）进行筛查。大多数开展ANCA检测的实验室使用商业试剂盒。尽管常规临床实验室不鼓励采用内部检测方法，但了解该检测方法的方法学方面仍然很重要。这些方面包括底物的选择、固定剂的选择、染色程序和解读程序。本文提供了该方法的详细信息，并结合临床应用进行了讨论。