School of Management and Economics, Beijing Institute of Technology, Beijing 100081, China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Carbohydr Polym. 2019 Feb 15;206:557-564. doi: 10.1016/j.carbpol.2018.11.010. Epub 2018 Nov 10.
In the present study, an efficient ultrasonic/microwave-assisted extraction (UMAE) procedure for the polysaccharides from the fruit of Camptotheca acuminata (CAFP) was investigated and optimized. Under the optimum conditions (ratio of liquid to raw material 30 mL/g, microwave irradiation time of 20 min, microwave irradiation power of 570 W and a fixed ultrasonic power of 50 W obtained by the response surface analysis with Box-Behnken design, satisfactory yields of CAFP (6.81 ± 0.04%) were achieved. The development UMAE technique produced higher yields in a shorter time than conventional hot water extraction (HWE): 20 vs. 120 min. In addition, in vivo CAFP at suitable dose is effective on H22 murine hepatoma strains, and CAFP significantly inhibited the proliferation of human oral carcinoma KB, pancreatic carcinoma BXCP-3 and gastric carcinoma SGC-7901 cells in vitro, indicating CAFP might be suitable for nature antitumor therapeutic agent development.
在本研究中，考察并优化了一种从喜树果实中提取多糖的高效超声/微波辅助提取（UMAE）方法。在最佳条件下（料液比为 30 mL/g、微波辐射时间 20 min、微波辐射功率 570 W 和固定超声功率 50 W，通过 Box-Behnken 设计的响应面分析得到），喜树果实多糖（CAFP）的收率（6.81±0.04%）令人满意。与传统热水提取（HWE）相比，开发的 UMAE 技术在更短的时间内产生了更高的收率：20 分钟对 120 分钟。此外，合适剂量的体内 CAFP 对 H22 小鼠肝癌株有效，CAFP 显著抑制人口腔癌 KB、胰腺癌 BXCP-3 和胃癌 SGC-7901 细胞的体外增殖，表明 CAFP 可能适合开发天然抗肿瘤治疗剂。
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