Department of Growth and Reproduction and EDMaRC, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100 Copenhagen, Denmark.
Department of Growth and Reproduction and EDMaRC, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100 Copenhagen, Denmark.
Environ Int. 2019 Feb;123:318-324. doi: 10.1016/j.envint.2018.11.047. Epub 2018 Dec 14.
Biobank serum samples from longitudinal mother-child cohorts have been used to estimate prenatal exposures to endocrine disrupting chemicals (EDCs). However, the knowledge about variations in serum concentrations of non-persistent chemicals during pregnancy is limited.
To describe the within- and between-person variations in serum concentrations of non-persistent chemicals and changes over trimesters, including phthalate metabolites, parabens, phenols, and UV filters.
Longitudinal study with repeated blood samples from 128 healthy pregnant women during pregnancy.
Population based study at a University Hospital in Copenhagen 1999-2001.
503 repetitive prenatal serum samples from 128 pregnant women taken at approximately gestational week 12, 20, 30 and 40 were analyzed for 7 UV filters, 32 metabolites of 15 phthalate diesters, 8 phenols and 7 parabens by LC-MS/MS.
Ten of 32 phthalate metabolites from six out of 15 phthalate diesters, two of seven parabens, two of eight phenols and three of seven UV filters were measurable in more than half of the serum samples. Of these chemicals, mono‑ethyl phthalate (MEP), mono‑iso‑nonyl phthalate (MiNP), mono‑iso‑decyl phthalate (MiDP), 4‑methylbenzophenone (4‑MBP), 4‑hydroxybenzoephenone (4‑HBP) and n‑propyl paraben (nPrP) had intra-class correlation coefficients (ICC) above 0.4 in both adjusted and unadjusted analyses (0.427-0.795), indicating low within-person variation. The serum concentration of UV filters 4‑MBP and 4‑HBP significantly increased throughout pregnancy, also after adjusting for seasonal variation (4‑HBP: effect estimates 0.142-0.437, p < 0.001. 4‑MBP: effect estimates 0.156-0.458, p < 0.002.).
MEP, MiNP, MiDP, 4‑MBP, 4‑HBP and nPrP were measurable in >50% of serum samples and showed low within-person variation. Thus, it is possible with acceptable accuracy to evaluate maternal exposure during pregnancy for these non-persistent chemicals using one or more biobank serum samples. The here presented adjusted ICC values can in addition be applied as adjustment of residual variation in future studies that evaluate outcomes related to prenatal exposures.
从纵向母婴队列的生物库血清样本中可以估计内分泌干扰化学物质（EDC）的产前暴露情况。然而，关于怀孕期间非持久性化学物质血清浓度的变化知之甚少。
描述非持久性化学物质血清浓度的个体内和个体间变化以及在妊娠期间的 trimester 变化，包括邻苯二甲酸酯代谢物、对羟基苯甲酸酯、酚类和紫外线滤光剂。
对 1999-2001 年在哥本哈根一所大学医院进行的基于人群的研究中 128 名健康孕妇进行的纵向研究，在妊娠期间重复采集血样。
丹麦哥本哈根大学医院进行的基于人群的研究。
对 128 名孕妇在大约妊娠 12、20、30 和 40 周时采集的 503 份重复产前血清样本进行分析，采用 LC-MS/MS 分析 7 种紫外线滤光剂、15 种邻苯二甲酸酯二酯中的 32 种代谢物、8 种酚类和 7 种对羟基苯甲酸酯。
在 6 种邻苯二甲酸酯中二酯中的 32 种代谢物、7 种对羟基苯甲酸酯中的 2 种、8 种酚类中的 2 种和 7 种紫外线滤光剂中的 3 种可在一半以上的血清样本中检测到。在这些化学物质中，单乙基邻苯二甲酸酯（MEP）、单异壬基邻苯二甲酸酯（MiNP）、单异癸基邻苯二甲酸酯（MiDP）、4-甲基苯甲酮（4-MBP）、4-羟基苯甲酮（4-HBP）和正丙基对羟基苯甲酸酯（nPrP）在调整和未调整分析中的组内相关系数（ICC）均高于 0.4（0.427-0.795），表明个体内变异较低。紫外线滤光剂 4-MBP 和 4-HBP 的血清浓度在整个孕期呈上升趋势，即使在调整了季节性变化后也是如此（4-HBP：效应估计值 0.142-0.437，p<0.001. 4-MBP：效应估计值 0.156-0.458，p<0.002）。
MEP、MiNP、MiDP、4-MBP、4-HBP 和 nPrP 可在>50%的血清样本中检测到，且个体内变异较低。因此，使用一个或多个生物库血清样本，评估这些非持久性化学物质在怀孕期间的母体暴露情况是可能的，且具有可接受的准确性。此处呈现的调整 ICC 值可在未来评估与产前暴露相关的结果的研究中，作为残余变异的调整。
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